ExoCap™ Exosome Isolation and Enrichment Kits

Optimised for Serum, Plasma, and Cell Culture Supernatant.

What is ExoCap™?

ExoCap™ is a kit for exosome isolation and enrichment designed and optimised for use with serum, plasma and cell culture supernatants. This kit consists of magnetic particles coupled with antibodies that recognise the exosome surface antigens (CD9, CD63, CD81, or EpCAM), treatment buffer, washing/dilution buffer, and exosome elution buffer.

ExoCap™ uses functionalized Magnosphere™ magnetic microparticles for exosome separation. These beads are coated with a JSR Life Sciences proprietary polymer and cofactors which specifically bind to exosomes.

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Features and Advantages

Simple: Simple workflow, easy-to-follow with minimum hands-on time, protocol. Direct isolation possible, no front-end precipitation or ultracentrifugation needed.
Specific: Targets specific antigens for purification of a defined exosome population.
Versatile: Optimised for serum, plasma, and cell culture supernatant, with optimised protocols.
Intact: Exosomes can be eluted intact preserving internal cargo.

ExoCap™ Comparison Data

	ExoCap™ Kit	Ultracentrifuge
Method	Highly specific immunoprecipitation	Non-specific enrichment by centrifugation
Purity	High	Low
Samples	Optimized for serum, plasma, and cell culture supernatant	Cell culture supernatant, serum, plasma, urine
Equipment	Low-cost magnetic tube stand	Expensive ultracentrifuge
Minimum Sample Volume	100 μL	> 1 mL

ExoCap™ Performance Data

ExoCap™ enables isolation and enrichment of exosomal proteins.

Western blot of isolated exosomal proteins in Serum and Plasma. Comparing Ultracentrifugation, ExoCap CD9 kit, CD63 Kit, CD81 Kit, Composite kit, and EpCAM Kit — Isolated exosomal proteins from serum and plasma samples. Comparing Ultracentrifugation, ExoCap™ CD9 kit, ExoCap™ CD63 Kit, ExoCap™ CD81 Kit, ExoCap™ Composite kit, and ExoCap™ EpCAM Kit.

tighter size distribution characteristic of exosomes compared to precipitation methods — Particles isolated by ExoCap™ kits exhibit a tighter size distribution characteristic of exosomes compared to precipitation method.

ExoCap™ Citations

Woo JR et al. The Role of Isolation Methods on a Nanoscale Surface Structure and Its Effect on the Size of Exosomes. J Circ Biomark. 5, 11 (2016)
Heinzelman P et al. Nanoscale Extracellular Vesicle Analysis in Alzheimer’s Disease Diagnosis and Therapy. Int J Alzheimers Dis. 2016, 8053139 (2016)（PMID：27213078)
Freedman JE et al. Diverse human extracellular RNAs are widely detected in human plasma. Nat Commun. 7, 11106 (2016) （PMID： 27112789)
Patel RS et al. Adipose-derived stem cells from lean and obese humans show depot specific differences in their stem cell markers, exosome contents and senescence: role of protein kinase C delta (PKCδ) in adipose stem cell niche. Stem Cell Investig. 3, 2 (2016) （PMID：27358894)
Puig-Basagoiti F et al. Human Cathelicidin Compensates for the Role of Apolipoproteins in Hepatitis C Virus Infectious Particle Formation. J Virol. 90, 8464 (2016) （PMID：27440892)

Companion Products

ExoCap™ Nucleic Acid Elution Buffer is designed for use with ExoCap™ exosome isolation and enrichment kits for downstream nucleic acid extraction.

Read More about the ExoCap™ range from MBL International below:

Any questions? Get in touch!

MBL International products are exclusively distributed in the UK and Ireland by Caltag Medsystems Ltd.

ExoCap™ Exosome Isolation and Enrichment Kits