SARS-Cov-2 Neutralising Antibody Kits

When someone contracts COVID-19, the host’s immune response can be measured by the presence of neutralising antibodies. Neutralising antibodies prevent the interaction between SARS-CoV-2 virus and receptors on human cells, giving the patient immunity.

Neutralising antibody detection is therefore very important in understanding and treating COVID-19. For example, to:

Test neutralising antibody induction during vaccine development, and how long antibodies are present for after infection
Confirm COVID-19 patient immunity
Allow donor selection in convalescent plasma therapy (using the antibodies of immune patients to help patients with active COVID-19)
Identify compounds that can interfere with the mechanism of viral infection, to prevent or treat COVID-19

Read on to learn about neutralising antibodies, and new kits on the market to detect them!

COVID-19 Mechanism of Infection: RBD-ACE2 Complex

The mechanism of COVID-19 infection works through the interaction between the SARS-CoV-2 Spike protein, and the ACE2 receptor on the surface of human cells. The receptor binding domain (RBD) of the Spike protein binds to ACE2 receptors, allowing the virus to infect cells and to self-replicate.

MBL COVID infection mechanism

Neutralising antibodies bind to the RBD on the Spike protein, preventing the virus from binding to ACE2 receptors and infecting cells.

MBL neutralising antibodies

Neutralising Antibody Detection

Neutralising antibody detection kits work on the ELISA principle, normally, the reaction between Horseradish peroxidase (HRP) and colourmetric substrate. To detect Neutralising antibodies, either ACE2 or RBD is labelled with HRP, and the other is bound to a plate. In the example below, ACE2 is labelled.

If the donor sample contains neutralising antibodies, RBD and ACE2 will not bind together. ACE2 will be washed off, and there will be no signal

If there are no neutralising antibodies, RBD and ACE2 will bind and ACE2 will not be washed off. The HRP on ACE2 will then be able to react with the colourmetric substrate, producing a signal.

MBL neutralising antibody detection assay principle

SARS-CoV-2 Neutralisation Antibody Detection Kits from Caltag Medsystems

Caltag work with select partners from around the world to bring cutting edge products to the UK and European markets. Below are some of the new Neutralisation Antibody Detection Kits on the market, available from Caltag:

Adipogen Life Sciences: Highly Specific SARS-CoV-2 Neutralising Antibodies Detection Kit

A Specific & Fast Replacement of the Virus Neutralisation Test (vNT)

AdipoGen Life Sciences has developed a highly specific and fast SARS-CoV-2 Neutralizing Antibodies Detection Kit (Prod. No. AG-48B-0002) that can be used to detect the presence of neutralising antibodies against SARS-CoV-2 in serum/plasma in about 2 hours independently of species and isotypes!

The SARS-CoV-2 Neutralising Antibodies Detection Kit is an easy, safe, fast and scalable alternative to the classical neutralisation assays using Vero E6 cells, such as virus neutralisation test (vNT) and pseudo-virus neutralization test (pVNT) and does not need to follow biosafety requirements.

High Specificity, Reduce False Results

AdipoGen Life Sciences’ surrogate ELISA assay has been evaluated by the Bundeswehr Institute of Microbiology, München in comparison with the Genscript assay. Their conclusion is that, if samples are pre-screened by a conventional sensitive serological IgG ELISA, the AdipoGen neutralisation assay could replace virus neutralisation tests (NTs) as a subsequent confirmatory test due to its high specificity.

REFERENCE: Comparison of two commercial surrogate ELISAs to detect a neutralising antibody response to SARS-CoV-2: K.Mueller, et al.; SSRN Preprint (2020). Download ABSTRACT or PREPRINT PDF.

Useful Tool for SARS-CoV-2 Vaccine and Therapeutic Development:

This kit is administrable in vaccine and therapeutic development as it is suitable for all antibody isotypes and can be used to test the presence of neutralising antibodies in human and animal models. The kit is useful in current COVID-19 investigations of sero-prevalence, assessment of immune response, herd immunity, longevity of protective immunity, efficacy of different vaccine candidates as well as tracking infections in animals.

To Confirm & Characterize further SARS-CoV-2 Serological Assay Results:

This neutralising antibody detection kit is ideal to further characterize the positive anti-SARS-CoV-2 IgG antibodies, detected with the SARS-CoV-2 IgG (Spike RBD) Serological ELISA Kit (Prod. No. AG-45B-0020).

View Adipogen’s COVID-19 Products Brochure

MBL International: SARS-CoV-2 Neutralisation Antibody Detection Kit

MBL have already launched a simple SARS-CoV-2 Neutralisation Antibody Detection Kit in Japan, and are bringing it to the European market soon. Their assay offers simple and rapid testing with a time to results of up to 2 hours. As with Adipogen’s kit, MBL’s kit also does not use live virus, so is safe testing and can be performed in a normal laboratory.

Performance testing indicates a high correlation with results from a neutralisation test in which infective SARS-CoV-2 was used. This kits was endorsed by collaborators from the prestigious Keio University in Japan.

We are able to offer evaluation samples of this kit. Please contact us for details. Performance testing indicated a high correlation with results from a neutralization test in which infective SARS-CoV-2 was used.

View SARS-CoV-2 Neutralization Antibody Detection Kit Product Brochure

SARS-CoV-2 Neutralization Antibody Detection Kit
Product Code: 5360
Pack Size: 96 Wells

SARS-CoV-2 Anti-RBD Antibody Profiling Kit – Detect non-neutralising antibodies

Some anti-spike (RBD) antibodies neutralise the virus, while others do not. Among non-neutralising antibodies, there are some antibodies which enhance the severity of the disease. Therefore, knowing the type of antibodies in a sample is critical for the prevention and treatment of COVID19. The SARS-CoV-2 Anti-RBD Antibody Profiling Kit from MBL takes advantage of the interaction between RBD and ACE2 to distinguish between samples with no antibodies, neutralising antibodies, or the presence of non-neutralising antibodies.

View SARS-CoV-2 Anti-RBD Antibody Profiling Kit Product Brochure

SARS-CoV-2 Anti-RBD Antibody Profiling Kit
Product Code: 5370
Pack Size: 96 Wells

Abnova COVID-19 Neutralizing Antibody ELISA Kit

COVID-19 infection is the interaction between the SARS-CoV-2 Spike (S) protein’s RBD and the ACE2 receptor on the surface of human cells. Thus, the identification of small molecules, antibodies, or other biological molecules that interfere with the formation of the RBD-ACE2 complex could help to develop drugs to prevent or treat COVID.

Abnova’s COVID-19 Neutralizing Antibody ELISA Kit is an indirect enzyme-linked immunoassay (ELISA) for quantitative determination of COVID-19 neutralising antibody. Product code KA6111

View Abnova’s Neutralizing Antibody ELISA Kit Flyer

ElabScience Anti-SARS-CoV-2 Neutralization Antibody Test Kits

ElabScience offer 3 kits to detect neutralising antibodies:

Images courtesy of MBL and Adipogen. MBL, Adipogen, Abnova, and ElabScience products are distributed in the UK by Caltag Medsystems. For more information, please contact us.

SARS-Cov-2 Neutralising Antibody Kits