T cell-dependent OVA (ovalbumin) antigen is commonly used as a model protein for studying antigen-specific immune responses in mice. SIINFEKL is one peptide sequence of the OVA used to precisely probe various aspects of antigen presentation, including the kinetics of peptide generation, MHC class I surface stability, and presentation efficiency following pharmacological and genetic manipulations including high throughput drug screening. In addition, the SIINFEKL peptide in the presence of an adjuvant is used in mice models to determine the capacity to raise an immune response. Primary immunisation with the peptide may lead to memory T cell responses that generate strong recall responses which can be detected by staining with SIINFEKL (OVA)-tetramer. The percentage of OVA-specific CD8+ T cells indicates to the researchers the percentages of antigen-specific T cells and their effector phenotypes. The results would demonstrate the success of the vaccine against infectious diseases or cancer (1-4).
The H-2Kb OVA Tetramer detects OVA-specific CD8 T cells in C57BL/6 and other mouse strains expressing the class I allele, H-2Kb. Whereas I-Ab OVA-Specific detects CD4 T cells in I-A b mouse strains expressing the class II allele.
References:
- Inoue, Shinya, et al. “Induction of potent antitumor immunity by intradermal DNA injection using a novel needle‐free pyro‐drive jet injector.” Cancer Science 114.1 (2023): 34.
- Li, Yuanke, et al. “Tumor Cell Nanovaccines based on Genetically Engineered Antibody‐anchored Membrane.” Advanced Materials (2023): 2208923.
- Sen Chaudhuri, Arka, et al. “S100A4 exerts robust mucosal adjuvant activity for co-administered antigens in mice.” Mucosal Immunology 15.5 (2022): 1028-1039.
- Li, Hongjian, et al. “Circular RNA cancer vaccines drive immunity in hard-to-treat malignancies.” Theranostics 12.14 (2022): 6422.
OVA Related Products
| Product Code | Product Name | Target | Size |
|---|---|---|---|
| MBL-TS-M541-1 | H-2Kb OVA E1 Tetramer-EIINFEKL-PE | OVA | 50 tests |
| MBL-TS-M541-2 | H-2Kb OVA E1 Tetramer-EIINFEKL-APC | OVA | 50 tests |
| MBL-TS-M542-1 | H-2Kb OVA G4 Tetramer-SIIGFEKL-PE | OVA | 50 tests |
| MBL-TS-M542-2 | H-2Kb OVA G4 Tetramer-SIIGFEKL-APC | OVA | 50 tests |
| MBL-TS-M543-1 | H-2Kb OVA Q4H7 Tetramer-SIIQFEHL-PE | OVA | 50 tests |
| MBL-TS-M543-2 | H-2Kb OVA Q4H7 Tetramer-SIIQFEHL-APC | OVA | 50 tests |
| MBL-TS-M710-1 | T-Select I-Ab OVA 323-339 Tetramer-PE | OVA | 20 Tests |
| MBL-TS-M710-2 | T-Select I-Ab OVA 323-339 Tetramer-APC | OVA | 20 Tests |
| MBL-TB-5001-1 | iTAg Tetramer/PE – H-2 Kb OVA (SIINFEKL) | OVA | 50 tests |
| MBL-TB-5001-2 | iTAg Tetramer/APC – H-2 Kb OVA (SIINFEKL) | OVA | 50 tests |
| MBL-TB-5001-4 | Tetramer/BV421 – H-2 Kb OVA (SIINFEKL) | OVA | 50 tests |
| MBL-TS-M703-1 | T-Select I-Ad OVA323-339 Tetramer-PE | OVA | 20 Tests |
| MBL-TS-M703-2 | T-Select I-Ad OVA323-339 Tetramer-APC | OVA | 20 Tests |
Information provided by MBL.
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