What are Polyclonal Antibodies?
Polyclonal antibodies are a mixture of antibodies from multiple B-cell clones of a host animal that can recognise multiple epitopes on an antigen. Their unique binding characteristic to different epitopes can help amplify their signal, leading to increased sensitivity and better detection. Unlike monoclonal antibodies, created by selecting a single B-cell clone, polyclonal antibodies occur naturally through the immune response. Numerous applications, including Western Blot, ELISA, immunohistochemistry, and immunoprecipitation, can be carried out using polyclonal antibodies. Polyclonal antibody therapeutics are also commonly utilised in medicine for virus and toxin neutralisation and immunoglobulin replacement therapy (3). In diagnostics, polyclonal antibodies are used for pathology and lateral flow tests, among others.
There are known limitations, such as limited supply and off-target binding of these antibodies. New technologies readily overcome these issues, such as stringent validation procedures to maximise lot-to-lot reproducibility. With innovative methods available to overcome the limitations of polyclonal antibodies, they remain excellent cost-effective tools for researchers, diagnostic developers & drug developers alike.
Benefits of Polyclonal Antibodies
- Clonal Properties:
- Due to their ability to detect several target protein epitopes, polyclonal antibodies have a high sensitivity to the target protein.
- Biophysical Diversity:
- Polyclonal antibodies are more stable than other antibodies when environmental factors cause other antibodies’ inactivation, lability, or precipitation.
- Provides researchers with modification opportunities, including:
- Host species.
- Antigen quantity.
- Amount and distribution of injection sites.
- Antigen injection frequency.
- Adjuvant type.
- Adjuvant quantity.
- Quicker Production timeline.
- Development and production are done in parallel.
- Competitive pricing.
Check out the complete catalogue of polyclonal antibodies for various applications, from ProSci!
Customise Your Research with Polyclonal Antibody Services!
ProSci’s service team can assist you with designing, purifying, and producing custom polyclonal antibodies.
- Expertise in immunogen determination and peptide design.
- Isolation of spleen and splenocytes to convert a rabbit polyclonal to monoclonal.
- Provision of organs as a deliverable.
- Multiple Ph.D. scientists with specialised knowledge and experience.
- Exceptional customer service.
- Customisable deliverables tailored to meet specific needs.
- Custom schedules are available to fit any timeline.
At ProSci, these antibodies are generated in various host species from customised immunogens designed by expert antibody scientists. Their experts have years of experience designing antigens for small peptides, large proteins, small molecules, and special modifications such as post-translational modifications (PTMs), including phosphorylation and glycosylation. They carefully pair antigen designs to host animals, paying close attention to your desired final application to maximise success. ProSci offers Polyclonal Antibody Services in various custom configurations and standard packages, from Antigen Design and Synthesis to Immunisation to Production. See their Polyclonal Antibody Services here.
Does your project require polyclonal antibodies? ProSci’s antibody experts and skilled project managers are dedicated to saving you time and costs and optimising your project to accelerate your next breakthrough. Partner with ProSci and research confidently.
Caltag Medsystems is here to support you every step of the way through your custom project with ProSci.
References
- Bordeaux J, Welsh AW, Agarwal S, et al. Antibody validation. BioTechniques. 2010;48(3):197-209. doi:https://doi.org/10.2144/000113382
- Lipman NS, Jackson LR, Trudel LJ, Weis-Garcia F. Monoclonal Versus Polyclonal Antibodies: Distinguishing Characteristics, Applications, and Information Resources. ILAR Journal. 2005;46(3):258-268. doi:https://doi.org/10.1093/ilar.46.3.258
- Pelletier, J. P. R., & Mukhtar, F. (2020). Passive Monoclonal and Polyclonal Antibody Therapies. Immunologic Concepts in Transfusion Medicine, 251–348. https://doi.org/10.1016/B978-0-323-67509-3.00016-0
- Wootla, B., Denic, A., & Rodriguez, M. (2014). Polyclonal and monoclonal antibodies in clinic. Methods in molecular biology (Clifton, N.J.), 1060, 79–110. https://doi.org/10.1007/978-1-62703-586-6_5
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