Reagents based on MagnetofectionTM:
PolyMag
PolyMag Transfection Reagent has been optimised for use with all types of nucleic acids. This reagent is composed of magnetic nanoparticles coated with cationic molecules. It has been successfully tested on a large range of primary cells, hard-to-transfect cells and cell lines and its efficiency has been reported in many publications.
- Non-toxic.
- Serum-compatible.
- Simple, rapid and easy to use.
- For all types of nucleic acids.
PolyMag Neo Transfection Reagent is specifically designed to achieve high transfection efficiency combined with superior transgene expression level. It is composed of magnetic nanoparticles coated with cationic molecules. PolyMag Neo is the ideal transfection reagent for a wide variety of cells.
- High transgene expression.
- High transfection efficiency.
- Multipurpose (various types of nucleic acids).
- Simple, ready-to-use and rapid.
- Non-toxic.
- Compatible with and without serum-containing culture media.
PolyMag Neo can also be used for Genome Edition using the CRISPR-Cas9 system (see Application Note in Technical Resources). It is design for the cotransfection with 2 plasmids encoding guide RNA and Cas9 endonuclease.
CombiMag
CombiMag Transfection Reagent is based on using Magnetofection™ to improve transfection reagent efficiency. It has been designed to be used with any commercial transfection reagent and can be used with all types of nucleic acids. It has been successfully tested on a broad range of primary cells, hard-to-transfect cells, and cell lines. CombiMag allows creating your own optimal delivery system with at least 30% transfection efficiency enhancement.
- Ideal to enhance any transfection reagent efficiency without changing your protocol /cells.
- Non-toxic.
- Compatible with and without serum.
- Simple, rapid and easy to use.
- For all types of nucleic acids.
Magnetofectamine O2
Magnetofectamine O2 is a transfection kit designed for primary and hard-to-transfect cells. The alliance of MTX transfection reagent and CombiMag leads to increased transfection efficiency, minimised toxicity, and enhanced gene expression.
- Less nucleic acids used – minimised toxicity.
- No need to change your standard protocol.
- Simple, ready-to-use and rapid.
- Serum compatible and applicable to all nucleic acids.
LipoMag
LipoMag Transfection Kit combines OZ Biosciences’ DreamFect™ Gold, with CombiMag magnetic nanoparticles. By taking advantage of both Lipofection and Magnetofection, the LipoMag Kit reaches higher transfection efficiency, especially in primary cells, while reducing toxicity. It is suitable for all types of nucleic acids, including plasmid DNA, linearised DNA, siRNA, oligonucleotides, double-stranded RNA, mRNA, and shRNA.
- Highest efficiency without toxicity.
- Enhancement of DreamFect™ Gold efficiency and outperforming competitors.
- Biodegradable.
- Multipurpose (various types of nucleic acids).
- Simple, ready-to-use and rapid.
- Serum compatible.
NeuroMag
NeuroMag Transfection Reagent is the first dedicated Magnetofection™ transfection reagent for neurons from 1 DIV to 21 DIV. It has proven to be efficient in transfecting a large variety of primary neurons such as cortical, hippocampal, dorsal root ganglion and motor neurons with all types of nucleic acids. Moreover, high transfection efficiency was also achieved in primary astrocytes, oligodendrocyte precursors or neural stem cells as well as other cell lines (C6, B65, PC12, N2A…). Due to its unique properties, NeuroMag allows following transfected neurons for several days.
- Non-toxic and completely biodegradable: high transfected neuron viability.
- Ready-to-use, straightforward and rapid.
- For all types of nucleic acids.
Glial-Mag
Glial-Mag Transfection Reagent is designed to reach optimal transfection efficiency with microglial cell lines and primary cells. Glial-Mag kit is the association of a specific magnetic nanoparticles’ formulation (Glial-Mag reagent), issued from Magnetofection™ technology and a booster (Glial-Boost) designed to enhance transfection efficiency.
- Low nucleic acid amount – minimised toxicity.
- High level of nucleic acid compaction.
- Easy and straightforward protocol.
- Compatible with any culture medium.
XPMag
XPMag Transfection Reagent is a magnetic nanoparticle formulation dedicated to gene transfection in organotypic cultures of explant by “Reverse Magnetofection”.
“Reverse Magnetofection” provides a novel and non-toxic strategy for nucleic acid-based gene therapy in the retina that can be translated to a wide variety of organ explants.
XPMag associated with Reverse Magnetofection allows the delivery of nucleic acids up to the deepest explant layers. The magnetic force guides and concentrates complexes of nucleic acids and XPMag magnetic nanoparticles from the culture media to the tissue. This method of transfection is fully biocompatible and does induce apoptosis or inflammatory reactions.
- Low nucleic acid amount – minimised toxicity.
- High level of nucleic acid compaction.
- Easy and straightforward protocol.
- Compatible with any type of explant.
Reagents based on Lipofection:
EcoTransfect
EcoTransfect Transfection Reagent is an economical Lipofection reagent for the transfection of popular cell lines. It is the perfect solution to quickly analyse the biological activity of nucleic acids, to perform routine transfection assays at low cost and to accomplish high throughput screening.
- Economical.
- Good efficiency.
- Serum compatible.
- Simple, ready-to-use and rapid.
DreamFect™
DreamFect™ Transfection Reagent is based on an innovative biochemical method. It contains both a lipidic part and a cationic part allowing the association of nucleic acids with this transfection reagent and their delivery inside cells. Due to its unique properties, DreamFect™ releases large DNA amounts in the cytosol increasing DNA nuclear delivery (TEE Technology). Consequently, DreamFect™ is highly efficient at low doses of nucleic acids. Therefore, less cationic lipids are used and the potential secondary effects are avoided.
- Broad range of cell types.
- For all nucleic acid applications.
- Non-toxic.
- Serum compatible.
- Simple, rapid and easy to use.
DreamFect™ Gold Transfection Reagent allows transfection of all types of nucleic acids with a very high efficiency. Based on the Tee-technology, DreamFect™ Gold delivers a large quantity of nucleic acids leading to higher protein expression compared to other transfection reagents. It is fully biodegradable, does not interfere with cellular mechanisms, maintains high cell viability in every experiment and avoids any potential secondary effects.
- Superior protein expression level & delivery of nucleic acids.
- Transient and stable transfection.
- Serum compatible.
- Simple, rapid and easy to use.
DreamFect™ Stem Transfection Reagent allows multipotent stem cell transfection, with high efficiency and very low toxicity. Its specific composition, based on the TEE-technology, allows transfecting embryonic and multipotent stem cells in presence of serum, maintaining their undifferentiated stage and their capacities to differentiate.
DreamFect™ Stem exhibits high transfection efficiency on human adult multipotent stem cells such as Mesenchymal Stem Cells, Adipose derived Stem Cells, Amniotic Fluid Stem Cells, and KG1a (leukemic stem-like cells) as well as embryonic stem cells.
- Minimised toxicity due to reagent biodegradability and low DNA amount required.
- Cell phenotype and differentiation potential are not affected.
- Quantum yield:0.79.
- Serum compatible.
- Ready-to-use and rapid (no specific buffer).
HeLaFect
HeLaFect Transfection Reagent is specifically developed for HeLa cell lineage transfection with high efficiency. HeLaFect is a lipid-based reagent based on the Tee-Technology (“Triggered Endosomal Escape”). The cationic design of this reagent allows high nucleic acid compaction for efficient transport into HeLa cells (more than 80% of transfected HeLa cells). This reagent is composed of biodegradable lipids leading to high viability and is ready-to-use.
- Low nucleic acid amount – minimised toxicity.
- Easy and straightforward protocol.
- Compatible with any culture medium: medium change not required.
VeroFect
VeroFect Transfection Reagent is a powerful transfection reagent designed to obtain highly efficient and reproducible transfection of Vero cells. It can be used for many applications such as stable and transient transfection and protein and viral production.
- Non-toxic.
- Serum compatible.
- Simple, ready-to-use and rapid.
FlyFectin™
FlyFectin™ Transfection Reagent is a reagent based on the Lipofection TEE-technology, specifically designed to obtain highly efficient and reproducible transfection of insect cells. It is adapted to the delivery of all types of nucleic acids. FlyFectin™ can be used for many applications including the production of recombinant proteins using a Baculovirus expression system.
FlyFectin™ has been effectively used with several insect cells: Ag55, Anso, Asd43, Bm5, Cl8, Cpp512, High5, IPBL-SF21, Kc167, Ld652, Mos20, S2, Sf9, SL-2, SL-3, SPC-SL52.
- Non-toxic.
- Serum compatible.
- Simple, ready-to-use and rapid.
Reagents based on Polyfection:
Helix-IN™
Helix-IN™ DNA Transfection Reagent creates new possibilities for addressing issues of classical transfection technologies. OZ Biosciences revolutionises Polyfection with the design of Helix-IN™, a novel patented Cationic Hydroxylated Amphiphilic Multi-block Polymer (CHAMP™ Technology). This novel bi-functional co-polymer is biocompatible, ionisable, pH responsive and biodegradable.
- Broad spectrum transfection reagent for cell lines and hard-to-transfect cells.
- High Intracellular Protein Production while preserving viability.
- High-secreted Protein Production while minimising cellular stress.
- Compatible with any culture medium.
Information provided by OZ Biosciences.
Caltag Medsystems is the distributor of OZ Biosciences products in the UK and Ireland. If you have any questions about these products, please contact us.