A recent study published in Cell Metabolism by the lab of Shin-Ichiro Ima (Washington University School of Medicine) shows that the circulating levels of extracellular nicotinamide phosphoribosyltransferase (eNAMPT), a key NAD+ biosynthetic enzyme in mammals, decline with age in mice and humans. When overexpressed eNAMPT increases NAD+ levels in multiple tissues, enhances their function and extends lifespan of mice. The majority of eNAMPT found in serum/plasma circulates in extracellular vesicles (EVs) and it is a necessity for eNAMPT to be in EVs to act on target cells. Detection of eNAMPT in this study is performed by western blotting using Adipogen Life Sciences’ monoclonal antibody anti-Nampt (Visfatin/PBEF), mAb (OMNI379) (Prod. No. AG-20A-0034).
AdipoGen Life Sciences provides best reagents to study NAMPT in vitro and in vivo in human or mouse:
– Nampt (Visfatin/PBEF) (human) (rec.) (Prod. No. AG-40A-0031Y)
– Nampt (Visfatin/PBEF) (mouse) (rec.) (Prod. No. AG-40A-0056Y)
– Nampt (Visfatin/PBEF) (rat) (rec.) (Prod. No. AG-40A-0058)
– Nampt (Visfatin/PBEF) (human) ELISA Kit (Prod. No. AG-45A-0006Y)
– Nampt (Visfatin/PBEF) (mouse/rat) Dual ELISA Kit (Prod. No. AG-45A-0007Y)
LIT: Extracellular Vesicle-Contained eNAMPT Delays Aging and Extends Lifespan in Mice: M. Yoshida, et al.; Cell Metabol. (ePub ahead of print) (2019)