Renal proximal tubular epithelial cells (PTEpiC) play a crucial role in renal function. They reabsorb nearly all of the glucose and amino acids in the glomerular filtrate, while allowing other substances of no nutritional value to be excreted in the urine. PTEpiC are located near injury sites in a variety of congenital, metabolic, and inflammatory diseases. PTEpiC can produce inflammatory mediators such as cytokines and chemokines and actively participate in acute inflammatory processes by affecting and directing leukocyte chemotaxis via the production of IL-8 [1, 2]. PTEpiC express IL-2R alpha and MHC class II antigens during inflammation, after renal transplantation, or in crescentic glomerulonephritis [3]. PTEpiC culture provides a useful in vitro model for studying the relationship between tubular cells and renal disease.
HRPTEpiC from ScienCell Research Laboratories are isolated from human kidneys. HRPTEpiC (Product Code: SC-4100) are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. HRPTEpiC are characterised by immunofluorescence with antibodies specific to cytokeratin-18, -19 and vimentin. HRPTEpiC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HRPTEpiC are guaranteed to further expand for 15 population doublings under the conditions provided by ScienCell Research Laboratories.
Recommended Medium
It is recommended to use Epithelial Cell Medium (EpiCM, Cat. Product Code: SC-4101) for the culturing of HRPTEpiC in vitro.
Caltag Medsystems is the distributor of ScienCell products in the UK and Ireland. If you have any questions about these products, please contact us.
Publications
1.) Van der Hauwaert C, Savary G, Gnemmi V, Glowacki F, Pottier N, Bouillez A, Maboudou P, Zini L, Leroy X, Cauffiez C, Perrais M, Aubert S. (2013) “Isolation?and?characterization?of a?primary?proximal?tubular?epithelial cell?model?from?human kidney?by?CD10/CD13?double?labeling.” PLoS One.?8: e66750.
2.) Du T, Zou X, Cheng J, Wu S, Zhong L, Ju G, Zhu J, Liu G, Zhu Y, Xia S. (2013) “Human Wharton?s jelly-derived mesenchymal stromal cells reduce renal fibrosis through induction of native and foreign hepatocyte growth factor synthesis in injured tubular epithelial cells.” Stem Cell Res Ther. 4: 59.
3.) Dudas PL, Sague SL, Elloso MM, Farrell FX. (2011) “Proinflammatory/Profibrotic Effects of Interleukin-17A on Human Proximal Tubule Epithelium.” Nephron Exp Nephrol. 117: e114-23.
4.) Zhang H, Tasnim F, Ying JY, Zink D. (2009) “The impact of extracellular matrix coatings on the performance of human renal cells applied in bioartificial kidneys.”Biomaterials. 30: 2899-911.
5.) Hatsukari I, Singh P, Hitosugi N, Messmer D, Valderrama E, Teichberg S, Chaung W, Gross E, Schmidtmayerova H, Singhal PC. (2007) “DEC-205-mediated internalization of HIV-1 results in the establishment of silent infection in renal tubular cells.” J Am Soc Nephrol. 18: 780-7.