Inflammasomes are implicated in physiological and pathological inflammation including neurodegenerative disorders such Parkinson or Alzheimer diseases. Divalent manganese (Mn2+) activates chronic inflammation leading to increase of neurotoxicity. Recently, the lab of A.G. Kanthasamy (Iowa State University, US) showed that Mn2+ activates the inflammasome protein NLRP3 through mitochondrial dysfunction in microglial cells, consequently promoting neuroinflammation. Interestingly, it was observed in the same study that Mn2+ stimulates the release of the adaptor protein ASC in exosomes that can propagate inflammasome activation in neighboring cells.
Following standard inflammasome antibodies from AdipoGen Life Sciences are used in this study in western blot and immunocytochemistry applications:
– anti-Caspase-1 (p20) (mouse), mAb (Casper-1) (Prod. No. AG-20B-0042)
– anti-NLRP3/NALP3, mAb (Cryo-2) (Prod. No. AG-20B-0014)
– anti-Asc, pAb (AL177) (Prod. No. AG-25B-0006)
LIT: Manganese activates NLRP3 inflammasome signaling and propagates exosomal release of ASC in microglial cells: S. Sarkar, et al.; Science Signal. 12, 563 (2019)