AdipoGen Life Sciences‘ SARS-CoV-2 Spike Protein (D614G) (Stable Trimer) (rec.) (His) (Prod. No. AG-40B-6003) is a full-length soluble SARS-CoV-2 Spike protein (aa 16-1213) including a foldon trimerisation motif, mutated Furin recognition site (R682S, R685S) and two stabilising mutations (K986P and V987P). This protein has a His-tag fused to its C-terminus. This protein binds to the human SARS-CoV-2 receptor ACE2 and it binds to anti-SARS-CoV-2 Spike antibodies in serum or plasma. It is useful for structural biology research, vaccine development, serological diagnostic kit development and neutralising antibody screening.
Figure: Full-length trimeric SARS-CoV-2 Spike (Prod. No. AG-40B-6003) binds to its receptor ACE2 (human) (rec.) (Prod. No. AG-40B-0192) with high affinity.
Method: SARS-CoV-2 Spike Protein (D614G) (Stable Trimer) (rec.) (His) (Prod. No. AG-40B-6003) or SARS-CoV-2 Spike Protein S1 (RBD) (rec.) (His) (Prod. No. AG-40B-0195) are coated at 50nM overnight at 4°C. ACE2 (human) (rec.) (Prod. No. AG-40B-0192) is added (starting at a concentration of 8000 ng/ml with a twofold serial dilution) during one hour at RT and the interaction is then detected using an anti-Flag (HRP).
Figure: SARS-CoV-2 Spike Protein (D614G) (Stable Trimer) (rec.) (His) (Prod. No. AG-40B-6003) migrates by Size Exclusion Chromatography (SEC) as a single peak.
Method: SARS-CoV-2 Spike Protein (D614G) (Stable Trimer) (rec.) (His) has been injected in a SEC column ENrich SEC 650 10 x 300 mm (Bio-Rad).
Originally posted on adipogen.com/covid-19
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