Without stabilisation, flow cytometric analysis of blood samples must be performed within 48 hours of venepuncture, as apoptosis and proteolytic degradation of cellular markers will detrimentally affect the immunophenotypic profile. Blood samples older than 48 hours are often no longer
Classical Media vs Specialty Media
For decades, researchers have been culturing cells to study normal and diseased biological processes. Many of these studies use immortalized cell lines which proliferate indefinitely in culture. Immortalized cells thrive in classical media formulated with the minimum requirements for cell
Human and Animal Tissue Procurement
Caltag Medsystems have established agreements to procure both human and animal tissues. We have access to a wide range of human tissues that are obtained via the gift of tissue and organ donation for research advancements in medical science. All
Diaclone ELISpot: Ex-vivo Analysis of Protein Secretion
The Enzyme Linked Immunospot (ELISpot) Assay is a versatile and highly sensitive method for ex-vivo analysis of protein secretion post cell stimulation. Virtue of its high sensitivity (1 cell in 100,000) the ELISpot assay enables frequency analysis of activated cell
Applications for Single Domain Antibody
The single-domain antibodies (sdAb), lack light chains, yet possess unique characteristics providing researchers a myriad of options not readily available with conventional antibodies. In comparison, the sdAbs boast significantly higher stability and are much smaller in size, only 15kd. As
Solutions for Liver Metabolism Research
Caltag Medsystems offers many solutions for researchers working in the toxicology field. Research areas include: in-vitro ADME applications, innovative modes of culture, hepatotoxicity screening and mechanistic testing applications, infectious disease and drug metabolism studies. We provide the HepaRG cell line
Expansion and Cryopreservation of Primary Cells
Continually expanding our offering of products and services to researchers, Caltag Medsystems’ team of scientists specialise in the expansion and cryopreservation of human cells. • Large scale expansion • Allows streamlining of assay workflow • Cryopreservation in convenient size aliquots
White Paper: Role of the autophagy regulator Rubicon in the pathogenesis of fatty liver disease
In 2016, Dr. Tanaka, Dr. Yoshimori, and their colleagues clarified that increased expression of Rubicon in liver causes fatty liver. This was the first report to demonstrate fatty liver is caused by suppression of autophagy in an acquired environment. In this
White Paper: Analysis of neoantigen-reactive T cells using the QuickSwitch™ custom tetramer kit
Kazuhiro Kakimi MD, PhD has been a leader in developing effective immunotherapies against cancer for the last 15 years in Japan. In this whitepaper, he and his colleagues have reported that the QuickSwitch™ Custom Tetramers will become an essential tool
Evaluating the biological activity of a cancer vaccine with the HLA-A*02:01 QuickSwitch™ Quant platform
Principle of the cell-based T2 shift assay and the cell-free QuickSwitch™ Quant assay Comparison of the cell-based T2 shift assay and the cell-free QuickSwitch™ Quant assay Immuno-oncology is a growing field in which different strategies such as immune modulators, vaccines