Kazuhiro Kakimi MD, PhD has been a leader in developing effective immunotherapies against cancer for the last 15 years in Japan. In this whitepaper, he and his colleagues have reported that the QuickSwitch™ Custom Tetramers will become an essential tool
Evaluating the biological activity of a cancer vaccine with the HLA-A*02:01 QuickSwitch™ Quant platform
Principle of the cell-based T2 shift assay and the cell-free QuickSwitch™ Quant assay Comparison of the cell-based T2 shift assay and the cell-free QuickSwitch™ Quant assay Immuno-oncology is a growing field in which different strategies such as immune modulators, vaccines
Antibodies for fluorescent proteins: Will it cross react with my variant?
Green fluorescent protein (GFP) was first isolated by Dr. Osamu Shimomura in 1961. Since then, fluorescent protein (FP) technology has made drastic advancements by many researchers. Currently, FP technology is the most popular tool for visualizing target proteins. Dr. Shimomura,
Mechanisms and detection methods of p62/SQSTM1 and its importance in the autophagy pathway.
Mechanisms of Autophagy The ubiquitin-conjugating protein p62/SQSTM1 is thought to be a scaffold protein that interacts with a variety of molecules involved in toll-like receptor (TLR) signaling, such as TRAF6, ERK, and aPKC. Recent evidence suggests that p62 binds directly
The Problem with Ultracentrifugation for Exosome Isolation
Ultracentrifugation is regarded as the gold standard method for exosome isolation. The ultracentrifugation process is time consuming and may introduce protein and nucleic acid contamination. Pre-clearing samples by one or more low speed centrifugation steps will deplete the cells, platelets,
Why Autophagy research won a Nobel Prize
The process of autophagy was first recognized in the late 1950s and it was thought of as bulk “junk” removal. Then, dedicated scientists did a little more digging and found there is an amazing methodology to the “junk” removal. It
Tools for Neurology Research
World Brain Day is annual initiative of the World Federation of Neurology. This year, on 22nd July, it was focused on Stroke prevention and treatment. In partnership with our global network of Life Science suppliers, we are pleased to bring
Why pure exosome isolation matters
Exosomes are cell-derived vesicles originating from multi-vesicular bodies and found in biological fluids such as blood, saliva, urine, and breast milk. Sizes of these extracellular vesicles (EVs) range between 30-100 nm. Due to their capacity to transfer proteins, lipids and
How to detect functional PCSK9 quickly and easily
PCSK9 remains an important target in cholesterol research. One of the hurdles in this research area is determining which PSCK9 form is present and binding with LDLR. MBL’s new Human PCSK9 Functional Assay Kit is designed to detect active PSCK9
High-Quality Stock and Custom Peptides
Suitable for Stimulation Assays High-Quality Control ≥95% Purity and Mass Spec Analysed Custom tetramer generation using QuickSwitch™ and QuickSwitch™ Quant Tetramer MBL International‘s stock and custom peptides can be used for stimulation assays along with custom tetramer generation using