Whenever our immune system is faced with a challenge, be it a multicellular parasite, a bacteria, a virus, or even cancer, this threat is detected and sampled primarily by the protein sequence of the pathogen in question. Other aspects of
Tips for Staining Rare Antigens with MHC Tetramers
When performing staining with MHC tetramers, there may be certain cases where the staining is unclear. An example could be in certain cases where an antigen-specific T cell population of interest has a low frequency. The following tips discussed in
Evaluating the biological activity of a cancer vaccine with the HLA-A*02:01 QuickSwitch™ Quant platform
Principle of the cell-based T2 shift assay and the cell-free QuickSwitch™ Quant assay Comparison of the cell-based T2 shift assay and the cell-free QuickSwitch™ Quant assay Immuno-oncology is a growing field in which different strategies such as immune modulators, vaccines
Help! My mouse tetramer stained ALL the CD8+ T cells!
You’re sitting at your flow cytometer, staring at your CD8 x tetramer plot in anticipation of a tiny but meaningful group of dots appearing in the upper right quadrant. You hold your breath, click “acquire,” and watch… Wait, what? What’s