When developing therapeutic monoclonal antibodies (mAbs), the choice of IgG subclass is especially important and needs to be considered. For example, IgG1 has the maximum potential to activate all 5 effector functions therefore, this isotype may be ideal for eliminating cancer tumours. Whereas, human IgG2 isotype is selected for neutralisation of antigens. By contrast, IgG3 is seldom used for therapeutic mAbs due to the long hinge region being prone to proteolysis resulting in a decreased half-life. Human IgG4 is distinct from all other IgG subclasses and antibody classes (IgA, IgM, IgE); since its Fc region does not activate a complement cascade, but still has other effector functions as indicated in Table 1. Currently, a substantial majority of approved functional grade antibodies in tumour therapy are of human IgG1 isotype. However, human IgG2 and IgG4 isotypes are increasingly used due to their non-Fc-mediated effector functions. An example of IgG4 usage is as a checkpoint inhibitor mAbs for which ADCC and CDC activities are preferentially avoided.
MBLI would like to offer you an insightful webinar that addresses the frequently asked questions they receive from researchers. Additionally, they have included valuable information to assist you in designing your in vivo studies.
Discover the profound impact of antibody selection on your experiment through this crucial webinar.
Or Read the White Paper below to gain in-depth knowledge of the importance of using isotype controls:
Highlights of the white paper Include:
- How to choose the appropriate control for any given in vivo study
- Why PBS is not a suitable substitute for an isotype control, with examples of how PBS use causes a range of issues which can hinder study performance and contribute to erroneous results
- Why isotype controls are also an ideal negative control for immunohistochemistry experiments
| Product Code | Product Name | Applications: |
| C0004 | In vivo Grade Human IgG4 kappa Isotype Control | ELISA, FACS, IF, IHC, in vivo studies, IP, WB |
| C0005 | In vivo Grade Mouse IgG1 kappa Isotype Control | ELISA, FACS, IF, IHC, in vivo studies, IP, WB |
| C0008 | In vivo Grade Mouse IgG2b kappa Isotype Control | ELISA, FACS, IF, IHC, in vivo studies, IP, WB |
Information provided by MBL.
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