Visualizing Cell Death Won’t Be Scary With MBLI Tunel Kit -MBL International

Programmed cell death is a selective process of physiological cell deletion. It plays a major role in developmental biology and in maintenance of homeostasis in vertebrates. For example, during maturation, T cells recognising self antigens are destroyed by programmed cell death. Programmed cell death is morphologically known apoptosis. Apoptosis is accompanied by condensation of cytoplasm, loss of plasma membrane microvilli, condensation and fragmentation of nuclei, and extensive degradation of chromosomal DNA into oligomers of about 180 bp. It has been considered that fragmentation of nuclear DNA is a biochemical hallmark of apoptosis. The TdT-mediated dUTP-biotin nick end labeling method (TUNEL method) enabled in situ visualization of DNA fragmentation at the single cell level and is considered to be a more sensitive method than conventional morphological techniques.

The MEBSTAIN Apoptosis TUNEL Kit Direct is an apoptosis detection kit based on the TUNEL method. The main advantage of this rapid and simple procedure is the use of fluorescein-dUTP to label DNA strand breaks. This allows the direct detection of DNA fragmentation.

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REFERENCES:

• 1. Kozako, Tomohiro, et al. “SRT1720 induces SIRT1‐independent cell death in adult T‐cell leukemia/lymphoma.” The FEBS Journal (2022).
• 2. Tsuji, Kohei, et al. “Suppression of non‐small‐cell lung cancer A549 tumor growth by an mtDNA mutation‐targeting pyrrole‐imidazole polyamide‐triphenylphosphonium and a senolytic drug.” Cancer science 113.4 (2022): 1321.
• 3. Nishio, Miki, et al. “Heterozygous loss of Zbtb38 leads to early embryonic lethality via the suppression of Nanog and Sox2 expression.” Cell proliferation 55.4 (2022): e13215.
• 4. Sanada, Yohei, et al. “Senescence-Accelerated Mice Prone 8 (SAMP8) in male as a spontaneous osteoarthritis model.” (2022).

For Research Use Only. Not for use in diagnostic procedures.

Originally posted by MBL International Corp