ProSci

TLR9 Antibody [TR9541]

Product Code:
 
PSI-33-002
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Mouse
Antibody Isotype:
 
IgG1, kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
TR9541
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Shipping:
 
blue ice
Storage:
 
Aliquot and Store at -20C. Avoid freez-thaw cycles.
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Western blot testing of human samples with TLR9 antibody at 2ug/ml.
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Western blot testing of human samples with TLR9 antibody at 2ug/ml.

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PSI-33-002-100ug100ug£662.00
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Further Information

Additional Names:
TLR9 Antibody: CD289, Toll-like receptor 9
Application Note:
Western blot: 2-4 ug/ml

Flow Cytometry: 1ug/10e6 cells

Immunohistochemistry (Paraffin): 3-5 ug/ml (1)

1. For optimal TLR9 staining, formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
Background:
The mammalian immune system is composed of two components: innate immunity and adaptive immunity. Toll-like receptors (TLRs) are an essential part of the innate immune system. Through recognition of pathogen-associated molecular patterns (PAMPs), TLRs mount an immune defense against pathogens. There are 10 mammalian TLRs (TLR1-10). Unmethylated CpG sequences in bacterial genomes and viral DNA molecules activates TLR9, leading to the inflammatory response.
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.5 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
A recombinant protein fragment from the N-terminal portion of human TLR9 was used as the immunogen for this antibody.
NCBI Gene ID #:
54106
NCBI Official Name:
toll-like receptor 9
NCBI Official Symbol:
TLR9
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Purification:
Protein G Affinity Chromatography
Research Area:
Signal Transduction,Innate Immunity
Swissprot #:
Q9NR96
User NOte:
Optimal dilutions for each application to be determined by the researcher