ProSci

HMGA1 Antibody

Product Code:
 
PSI-16-565
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
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Western blot analysis of extracts of 293T cells, using HMGA1 antibody (16-565) at 1:3000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit._Exposure time: 90s.
2 / 3
Immunohistochemistry of paraffin-embedded human stomach using HMGA1 antibody (16-565) at dilution of 1:100 (40x lens).
3 / 3
Immunofluorescence analysis of HeLa cells using HMGA1 antibody (16-565) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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Western blot analysis of extracts of 293T cells, using HMGA1 antibody (16-565) at 1:3000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit._Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human stomach using HMGA1 antibody (16-565) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using HMGA1 antibody (16-565) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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PSI-16-565-100uL100 uL£565.00
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Further Information

Additional Names:
nonhistone chromosomal high-mobility group protein HMG-I/HMG-Y, high-mobility group (nonhistone chromosomal) protein isoforms I and Y, OTTHUMP00000039618, OTTHUMP00000016224, OTTHUMP00000016223, OTTHUMP00000016222, MGC4854, MGC4242, MGC12816, HMGIY, HMGA1A, HMG-R, high mobility group AT-hook 1, HMGA1
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Background:
This gene encodes a chromatin-associated protein involved in the regulation of gene transcription, integration of retroviruses into chromosomes, and the metastatic progression of cancer cells. The encoded protein preferentially binds to the minor groove of AT-rich regions in double-stranded DNA. Multiple transcript variants encoding different isoforms have been found for this gene. Pseudogenes of this gene have been identified on multiple chromosomes.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human HMGA1 (NP_665908.1).
NCBI Gene ID #:
3159
NCBI Official Name:
high mobility group AT-hook 1
NCBI Official Symbol:
HMGA1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 20kDa
Purification:
Affinity purification
Research Area:
Cancer, Transcription
Swissprot #:
P17096
User NOte:
Optimal dilutions for each application to be determined by the researcher.