ProSci

CD86 Antibody

Product Code:
 
PSI-16-756
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
1 / 5
Western blot analysis of extracts of various cell lines, using CD86 antibody (16-756) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
2 / 5
Immunofluorescence analysis of THP-1 cells using CD86 Polyclonal Antibody (16-756) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
3 / 5
Immunofluorescence analysis of rat spleen using CD86 Polyclonal Antibody (16-756) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
4 / 5
Immunofluorescence analysis of mouse spleen using CD86 Polyclonal Antibody (16-756) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
5 / 5
Immunoprecipitation analysis of 200ug extracts of Jurkat cells using 3ug CD86 antibody (16-756). Western blot was performed from the immunoprecipitate using CD86 antibody (16-756) at a dilition of 1:1000.
❮❯

Western blot analysis of extracts of various cell lines, using CD86 antibody (16-756) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
Immunofluorescence analysis of THP-1 cells using CD86 Polyclonal Antibody (16-756) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of rat spleen using CD86 Polyclonal Antibody (16-756) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of mouse spleen using CD86 Polyclonal Antibody (16-756) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 200ug extracts of Jurkat cells using 3ug CD86 antibody (16-756). Western blot was performed from the immunoprecipitate using CD86 antibody (16-756) at a dilition of 1:1000.

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PSI-16-756-100uL100 uL£565.00
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Further Information

Additional Names:
CD86 Antibody: CD86 molecule, B70, B7-2, B7.2, LAB72, CD28LG2
Application Note:
WB: 1:500 - 1:2000

IF: 1:50 - 1:200

IP: 1:50 - 1:200
Background:
This gene encodes a type I membrane protein that is a member of the immunoglobulin superfamily. This protein is expressed by antigen-presenting cells, and it is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of this protein with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of this protein with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response. Alternative splicing results in several transcript variants encoding different isoforms.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant protein of human CD86.
NCBI Gene ID #:
942
NCBI Official Name:
CD86 molecule
NCBI Official Symbol:
CD86
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 80kDa
Purification:
Affinity purification
Research Area:
Immunology, Stem Cell
Swissprot #:
P42081
User NOte:
Optimal dilutions for each application to be determined by the researcher.