ProSci

PPP2CA Antibody

Product Code:
 
PSI-22-419
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
1 / 3
Western blot analysis of extracts of various cell lines, using PPP2CA antibody (22-419) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
2 / 3
Immunohistochemistry of paraffin-embedded human stomach using PPP2CA antibody (22-419) at dilution of 1:100 (40x lens).
3 / 3
Immunofluorescence analysis of HeLa cells using PPP2CA antibody (22-419). Blue: DAPI for nuclear staining.
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Western blot analysis of extracts of various cell lines, using PPP2CA antibody (22-419) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human stomach using PPP2CA antibody (22-419) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using PPP2CA antibody (22-419). Blue: DAPI for nuclear staining.

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PSI-22-419-100uL100 uL£565.00
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Further Information

Additional Names:
PPP2CA, PP2Ac, PP2Calpha, ppp2A1, Protein phosphatase 2A alpha, Replication protein C, PP2CA, RP-C, PP2A-alpha
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Background:
This gene encodes the phosphatase 2A catalytic subunit. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. This gene encodes an alpha isoform of the catalytic subunit.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-309 of human PPP2CA (NP_002706.1).
NCBI Gene ID #:
5515
NCBI Official Name:
protein phosphatase 2, catalytic subunit, alpha isozyme
NCBI Official Symbol:
PPP2CA
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 36kDa
Purification:
Affinity purification
Research Area:
Apoptosis, Cancer, Cell Cycle, Immunology, Signal Transduction
Swissprot #:
P67775
User NOte:
Optimal dilutions for each application to be determined by the researcher.