ProSci

FKBP3 Antibody

Product Code:
 
PSI-22-545
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
1 / 3
Western blot analysis of extracts of various cell lines, using FKBP3 antibody (22-545) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 30s.
2 / 3
Immunofluorescence analysis of HeLa cells using FKBP3 antibody (22-545). Blue: DAPI for nuclear staining.
3 / 3
Immunoprecipitation analysis of 200ug extracts of MCF-7 cells, using 3 ug FKBP3 antibody (22-545). Western blot was performed from the immunoprecipitate using FKBP3 antibody (22-545) at a dilition of 1:1000.

Western blot analysis of extracts of various cell lines, using FKBP3 antibody (22-545) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 30s.
Immunofluorescence analysis of HeLa cells using FKBP3 antibody (22-545). Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 200ug extracts of MCF-7 cells, using 3 ug FKBP3 antibody (22-545). Western blot was performed from the immunoprecipitate using FKBP3 antibody (22-545) at a dilition of 1:1000.

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PSI-22-545-100uL100 uL£565.00
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This product comes from: United States.
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Further Information

Additional Names:
FKBP3, 25 kDa FK506-binding protein, 25 kDa FKBP, FKBP-3, Immunophilin FKBP25, FK506-binding protein 3, FK506-binding protein 3 (25kD), PPIase FKBP3, Rapamycin binding protein, Rotamase, PPIase, FK506 binding protein 3, 25kDa, FKBP-25, FKBP25
Application Note:
WB: 1:500 - 1:2000

IF: 1:50 - 1:100

IP: 1:50 - 1:100
Background:
The protein encoded by this gene is a member of the immunophilin protein family, which play a role in immunoregulation and basic cellular processes involving protein folding and trafficking. This encoded protein is a cis-trans prolyl isomerase that binds the immunosuppressants FK506 and rapamycin, as well as histone deacetylases, the transcription factor YY1, casein kinase II, and nucleolin. It has a higher affinity for rapamycin than for FK506 and thus may be an important target molecule for immunosuppression by rapamycin.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-224 of human FKBP3 (NP_002004.1).
NCBI Gene ID #:
2287
NCBI Official Name:
FK506 binding protein 3, 25kDa
NCBI Official Symbol:
FKBP3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 30kDa
Purification:
Affinity purification
Research Area:
Other
Swissprot #:
Q00688
User NOte:
Optimal dilutions for each application to be determined by the researcher.