ProSci

CCAR2 Antibody

Product Code:
 
PSI-22-722
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
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Western blot analysis of extracts of various cell lines, using CCAR2 antibody (22-722) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
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Immunohistochemistry of paraffin-embedded rat brain using CCAR2 antibody (22-722) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded rat kidney using CCAR2 antibody (22-722) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human rectal cancer using CCAR2 antibody (22-722) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse liver using CCAR2 antibody (22-722) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse brain using CCAR2 antibody (22-722) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse kidney using CCAR2 antibody (22-722) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse stomach using CCAR2 antibody (22-722) at dilution of 1:100 (40x lens).
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Immunofluorescence analysis of U2OS cells using CCAR2 antibody (22-722). Blue: DAPI for nuclear staining.
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Western blot analysis of extracts of various cell lines, using CCAR2 antibody (22-722) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
Immunohistochemistry of paraffin-embedded rat brain using CCAR2 antibody (22-722) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat kidney using CCAR2 antibody (22-722) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human rectal cancer using CCAR2 antibody (22-722) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse liver using CCAR2 antibody (22-722) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using CCAR2 antibody (22-722) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using CCAR2 antibody (22-722) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse stomach using CCAR2 antibody (22-722) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using CCAR2 antibody (22-722). Blue: DAPI for nuclear staining.

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CodeSizePrice
PSI-22-722-100uL100 uL£565.00
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Further Information

Additional Names:
CCAR2, cell cycle and apoptosis regulator 2, DBC-1, DBC1, KIAA1967, NET35, p30 DBC, p30DBC, cell cycle and apoptosis regulator protein 2, cell division cycle and apoptosis regulator protein 2, deleted in breast cancer 1, p30 DBC protein
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 654-923 of human CCAR2 (NP_066997.3).
NCBI Gene ID #:
57805
NCBI Official Name:
cell cycle and apoptosis regulator 2
NCBI Official Symbol:
CCAR2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 100kDa
Purification:
Affinity purification
Research Area:
Apoptosis, Cancer, Cell Cycle
Swissprot #:
Q8N163
User NOte:
Optimal dilutions for each application to be determined by the researcher.