ProSci

CBR3 Antibody

Product Code:
 
PSI-23-018
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
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Immunofluorescence analysis of A549 cells using CBR3 antibody (23-018). Blue: DAPI for nuclear staining.
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Immunohistochemistry of paraffin-embedded mouse stomach using CBR3 antibody (23-018) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded rat brain using CBR3 antibody (23-018) at dilution of 1:100 (40x lens).
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Western blot analysis of extracts of various cell lines, using CBR3 antibody (23-018) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
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Immunofluorescence analysis of A549 cells using CBR3 antibody (23-018). Blue: DAPI for nuclear staining.
Immunohistochemistry of paraffin-embedded mouse stomach using CBR3 antibody (23-018) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using CBR3 antibody (23-018) at dilution of 1:100 (40x lens).
Western blot analysis of extracts of various cell lines, using CBR3 antibody (23-018) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.

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PSI-23-018-100uL100 uL£565.00
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Further Information

Additional Names:
CBR3, carbonyl reductase 3, carbonyl reductase (NADPH) 3, hCBR3, SDR21C2, HEL-S-25
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Background:
Carbonyl reductase 3 catalyzes the reduction of a large number of biologically and pharmacologically active carbonyl compounds to their corresponding alcohols. The enzyme is classified as a monomeric NADPH-dependent oxidoreductase. CBR3 contains three exons spanning 11.2 kilobases and is closely linked to another carbonyl reductase gene - CBR1.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 118-277 of human CBR3 (NP_001227.1).
NCBI Gene ID #:
874
NCBI Official Name:
carbonyl reductase 3
NCBI Official Symbol:
CBR3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 31kDa
Purification:
Affinity purification
Research Area:
Cancer, Signal Transduction
Swissprot #:
O75828
User NOte:
Optimal dilutions for each application to be determined by the researcher.