ProSci

GLUD1 Antibody

Product Code:
 
PSI-23-064
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
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Western blot analysis of extracts of various cell lines, using GLUD1 antibody (23-064) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 20s.
2 / 9
Immunohistochemistry of paraffin-embedded human liver cancer using GLUD1 antibody (23-064) at dilution of 1:100 (40x lens).
3 / 9
Immunohistochemistry of paraffin-embedded human colon carcinoma using GLUD1 antibody (23-064) at dilution of 1:100 (40x lens).
4 / 9
Immunohistochemistry of paraffin-embedded human esophagus using GLUD1 antibody (23-064) at dilution of 1:100 (40x lens).
5 / 9
Immunofluorescence analysis of C6 cells using GLUD1 antibody (23-064) at dilution of 1:100. Blue: DAPI for nuclear staining.
6 / 9
Immunofluorescence analysis of NIH-3T3 cells using GLUD1 antibody (23-064) at dilution of 1:100. Blue: DAPI for nuclear staining.
7 / 9
Immunofluorescence analysis of C6 cells using GLUD1 antibody (23-064) at dilution of 1:100. Blue: DAPI for nuclear staining.
8 / 9
Immunofluorescence analysis of NIH-3T3 cells using GLUD1 antibody (23-064) at dilution of 1:100. Blue: DAPI for nuclear staining.
9 / 9
Immunofluorescence analysis of U-2 OS cells using GLUD1 antibody (23-064) at dilution of 1:100. Blue: DAPI for nuclear staining.
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Western blot analysis of extracts of various cell lines, using GLUD1 antibody (23-064) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 20s.
Immunohistochemistry of paraffin-embedded human liver cancer using GLUD1 antibody (23-064) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human colon carcinoma using GLUD1 antibody (23-064) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human esophagus using GLUD1 antibody (23-064) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of C6 cells using GLUD1 antibody (23-064) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH-3T3 cells using GLUD1 antibody (23-064) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of C6 cells using GLUD1 antibody (23-064) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH-3T3 cells using GLUD1 antibody (23-064) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U-2 OS cells using GLUD1 antibody (23-064) at dilution of 1:100. Blue: DAPI for nuclear staining.

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PSI-23-064-100uL100 uL£565.00
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  • Further Information
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Further Information

Additional Names:
GLUD1, GDH1, GDH 1, Glutamate dehydrogenase 1, Glutamate dehydrogenase 2, GDH, GLUD
Application Note:
WB: 1:500 - 1:2000

IHC: 1:100 - 1:200

IF: 1:50 - 1:200
Background:
This gene encodes glutamate dehydrogenase, which is a mitochondrial matrix enzyme that catalyzes the oxidative deamination of glutamate to alpha-ketoglutarate and ammonia. This enzyme has an important role in regulating amino acid-induced insulin secretion. It is allosterically activated by ADP and inhibited by GTP and ATP. Activating mutations in this gene are a common cause of congenital hyperinsulinism. Alternative splicing of this gene results in multiple transcript variants. The related glutamate dehydrogenase 2 gene on the human X-chromosome originated from this gene via retrotransposition and encodes a soluble form of glutamate dehydrogenase. Related pseudogenes have been identified on chromosomes 10, 18 and X.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 54-240 of human GLUD1 (NP_005262.1).
NCBI Gene ID #:
2746
NCBI Official Name:
glutamate dehydrogenase 1
NCBI Official Symbol:
GLUD1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 50kDa
Purification:
Affinity purification
Research Area:
Cancer, Cell Cycle, Signal Transduction
Swissprot #:
P00367
User NOte:
Optimal dilutions for each application to be determined by the researcher.