Human BMP-7 ELISA Kit

Bone morphogenetic protein-7 or BMP-7 (also known as osteogenic protein-1 or OP-1) is a protein that in humans is encoded by the BMP7 gene. The protein encoded by this gene is a member of the TGF-β superfamily. Like other members of the bone morphogenetic protein family of proteins, BMP-7 plays a key role in the transformation of mesenchymal cells into bone and cartilage. It is inhibited by noggin and a similar protein, chordin, which are expressed in the Spemann-Mangold Organizer. BMP-7 may be involved in bone homeostasis. It is expressed in the brain, kidneys and bladder. ScienCell's human BMP-7 ELISA kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. Human BMP-7-specific monoclonal antibodies are pre-coated onto 8 x 12 divisible strips. The human specific detection polyclonal antibodies are biotinylated. The test samples and biotinylated detection antibodies are subsequently added to the wells and then washed with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes to yellow after adding acidic stop solution. The intensity of yellow is proportional to the amount of human BMP-7 in the sample that is captured on the strips.

For quantitative detection of human BMP-7 in bone tissue, cell culture supernatants, serum, plasma (heparin, EDTA) and urine.