Human CSF1R/M-CSFR ELISA Kit

CSF1R (Colony stimulating factor 1 receptor), also known as M-CSFR and CD115, is a cell-surface protein encoded, in humans, by the CSF1R gene. The gene is located on the long arm of chromosome 5 (5q32) on the Crick (minus) strand. The encoded protein is a tyrosine kinase transmembrane receptor and a member of the CSF1/PDGF receptor family of tyrosine-protein kinases. The protein is a single pass type I membrane protein and acts as the receptor for colony stimulating factor 1 (CSF1), a cytokine which controls the production, differentiation, and function of macrophages. Both CSF1R and its ligand CSF1 play an important role in the development of the mammary gland and may be involved in the process of mammary gland carcinogenesis. ScienCell's human CSF1R ELISA kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. Human CSF1R-specific polyclonal antibodies are pre-coated onto 8 x 12 divisible strips. The human-specific detection monoclonal antibodies are biotinylated. The test samples and biotinylated detection antibodies are subsequently added to the wells and then washed with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes to yellow after adding acidic stop solution. The intensity of yellow is proportional to the amount of human CSF1R in the sample that is captured on the strips.

For quantitative detection of human CSF1R in cell culture supernatants, serum and plasma (heparin, EDTA).