Human DKK-1 ELISA Kit

Dickkopf-related protein 1 (DKK-1) is a protein that in humans is encoded by the DKK1 gene, which is mapped to chromosome 10q11.2. This gene encodes a protein that is a member of the dickkopf family. It is a secreted protein with two cysteine-rich regions and is involved in embryonic development through its inhibition of the Wnt signaling pathway. Elevated levels of DKK-1 in bone marrow, plasma, and peripheral blood are associated with the presence of osteolytic bone lesions in patients with multiple myeloma. ScienCell's human DKK-1 ELISA kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. Human DKK-1-specific polyclonal antibodies are pre-coated onto 8 x 12 divisible strips. The human-specific detection monoclonal antibodies are biotinylated. The test samples and biotinylated detection antibodies are subsequently added to the wells and then washed with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes to yellow after adding acidic stop solution. The intensity of yellow is proportional to the amount of human DKK-1in the sample that is captured on the strips.

For quantitative detection of human DKK-1 in cell culture supernatants, serum and plasma (heparin, EDTA).