Human EG-VEGF ELISA Kit

EG-VEGF, endocrine gland-derived vascular endothelial growth factor, is a human protein encoded by the PROKR1 gene. It is mapped to chromosome 1. The 1.4kb cDNA encodes a protein consisting of 105 amino acids with a well defined signal sequence. The mature protein is predicted to have 86 amino acids. Northern blot analysis demonstrated expression in testis, ovary, adrenal gland, and placenta. EG-VEGF plays a vital role in inducing proliferation, migration, and fenestration in capillary endothelial cells derived from endocrine glands. The standard in this kit is recombinant human EG-VEGF (A20-F105), with molecular weight 10.5 kDa. ScienCell's human EG-VEGF ELISA kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. Human EG-VEGF-specific polyclonal antibodies are pre-coated onto 8 x 12 divisible strips. The human-specific detection polyclonal antibodies are biotinylated. The test samples and biotinylated detection antibodies are subsequently added to the wells and then washed with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes to yellow after adding acidic stop solution. The intensity of yellow is proportional to the amount of human EG-VEGF in the sample that is captured on the strips.

For quantitative detection of human EG-VEGF in cell culture supernatants, serum and plasma (heparin, EDTA).