Rat TGF-β1 ELISA Kit

Transforming growth factor-β1 (TGF-β1) is a multifunctional peptide that controls proliferation, differentiation, and other functions in many cell types. Many cells synthesize TGF-β and essentially all of them have specific receptors for this peptide. TGF-β regulates the actions of many other peptide growth factors and can have a positive or negative regulatory affect.1 TGF-β1 is known for its potent and diverse biological effects, including immune regulation, and cell growth and differentiation. TGF-β1 is also an important mediator of bone remodeling. TGF-β1, a potent keratinocyte growth inhibitor, has been shown to be overexpressed in keratinocytes in certain inflammatory skin diseases and has been thought to counteract the effects of other growth factors at the site of inflammation. TGF-β1, a multifunctional cytokine with fibrogenic properties, has been implicated in the pathogenesis of the vascular and target organ complications of hypertension. TGF-β1 may also regulate blood pressure via stimulation of endothelin-1 and/or renin secretion. TGF-β1 is secreted as a latent form, which consists of its mature form and a latency-associated peptide (β1-LAP) in either the presence or the absence of additional latent TGF-β1-binding protein. The standard product used in this kit is recombinant TGF-β1 with a molecular mass of 25kDa. ScienCell's rat TGF-β1 ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. Rat TGF-β1-specific polyclonal antibodies are pre-coated onto 8 x 12 strips. The rat-specific detection polyclonal antibodies are biotinylated. The test samples and biotinylated detection antibodies are subsequently added to the wells and then washed with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes to yellow after adding acidic stop solution. The intensity of yellow is proportional to the amount of rat TGF-β1 in the sample that is captured on the strips.

For quantitative detection of rat TGF-β1 in serum, body fluids, tissue lysates or cell culture supernatants.