ProSci

SARS-CoV-2 (COVID-19) NSP15 Antibody

Product Code:
 
PSI-9269
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Virus
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Shipping:
 
blue ice or RT
Storage:
 
SARS-CoV-2 (COVID-19) NSP15 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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<strong>Figure 1 ELISA Validation with SARS-CoV-2 (COVID-19) NSP15 Protein </strong><br> Antibodies: SARS-CoV-2 (COVID-19) NSP15 Antibody, 9269. A direct ELISA was performed using SARS-CoV-2 NSP15 recombinant protein (10-420) as coating antigen and the anti-SARS-CoV-2 (COVID-19) NSP15 antibody as the capture antibody. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:20000 dilution. Detection range is from 1 ng/mL to 3000 ng/mL
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<strong>Figure 2 Western Blot Validation with SARS-CoV-2 (COVID-19) NSP15 Protein</strong><br> Loading: 30 ng per lane of SARS-CoV-2 (COVID-19) NSP15 recombinant protein (10-420). Antibodies: SARS-CoV-2 (COVID-19) NSP15, 9269, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane 1: 0.5 μg/mL and Lane 2: 1 μg/mL
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<strong>Figure 1 ELISA Validation with SARS-CoV-2 (COVID-19) NSP15 Protein </strong><br> Antibodies: SARS-CoV-2 (COVID-19) NSP15 Antibody, 9269. A direct ELISA was performed using SARS-CoV-2 NSP15 recombinant protein (10-420) as coating antigen and the anti-SARS-CoV-2 (COVID-19) NSP15 antibody as the capture antibody. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:20000 dilution. Detection range is from 1 ng/mL to 3000 ng/mL
<strong>Figure 2 Western Blot Validation with SARS-CoV-2 (COVID-19) NSP15 Protein</strong><br> Loading: 30 ng per lane of SARS-CoV-2 (COVID-19) NSP15 recombinant protein (10-420). Antibodies: SARS-CoV-2 (COVID-19) NSP15, 9269, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane 1: 0.5 μg/mL and Lane 2: 1 μg/mL

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PSI-9269-0.02mg0.02mg£150.00
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PSI-9269-0.1mg0.1mg£449.00
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Further Information

Additional Names:
Uridylate-specific endoribonuclease, NendoU, NSP15, Non-structure protein 15
Application Note:
WB: 0.5-1 μg/mL

Antibody validated: SARS-CoV-2 (COVID-19) NSP15 antibody can detect 2 ng of free peptide at 1 μg/mL in ELISA. It can detect SARS-CoV-2 NSP15 recombinant protein by ELISA and WB. All other applications and species not yet tested.
Background:
Coronavirus disease 2019 (COVID-19), formerly known as 2019-nCoV acute respiratory disease, is an infectious disease caused by SARS-CoV-2, a virus closely related to the SARS virus (1). The disease is the cause of the 2019?20 coronavirus outbreak (2). The structure of 2019-nCoV consists of the following: a spike protein (S), hemagglutinin-esterease dimer (HE), a membrane glycoprotein (M), an envelope protein (E) a nucleoclapid protein (N) and RNA. NSP15 is a Mn2+-dependent, uridylate-specific enzyme, which leaves 2'-3'-cyclic phosphates 5' to the cleaved bond.
Background References:
  • Gorbalenya. bioRxiv: 2020.
  • Hui et al. Int J Infect Dis. 2020;91:264-266.
Buffer:
SARS-CoV-2 (COVID-19) NSP15 antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted reactivity based on immunogen sequence: SARS-CoV NSP15 protein: (100%).
Immunogen:
Anti-SARS-CoV-2 (COVID-19) NSP15 antibody (9269) was raised against a peptide corresponding to 15 amino acids near the carboxyl terminus of SARS-CoV-2 (COVID-19) NSP15 protein.

The immunogen is located within the last 50 amino acids of the SARS-CoV-2 (COVID-19) NSP15 protein.
NCBI Gene ID #:
43740578
NCBI Official Name:
ORF1a polyprotein;ORF1ab polyprotein
NCBI Official Symbol:
ORF1ab
NCBI Organism:
Severe acute respiratory syndrome coronavirus 2
Physical State:
Liquid
Protein Accession #:
6VWW_B
Protein GI Number:
1851775710
Purification:
SARS-CoV-2 (COVID-19) NSP15 Antibody is affinity chromatography purified via peptide column.
Research Area:
Infectious Disease,COVID-19
Swissprot #:
P0DTC1
User NOte:
Optimal dilutions for each application to be determined by the researcher.