Further Information
ORF3a protein, Accessory protein 3a, Protein 3a, Protein U274, Protein X1, ORF3a
IHC: 0.2 μg/mL
Antibody validated: Immunohistochemistry in human samples. Antibody validated: SARS-CoV-2 (COVID-19) ORF3a antibody can detect 2 ng of free peptide at 1 μg/mL in ELISA. All other applications and species not yet tested.
Coronavirus disease 2019 (COVID-19), formerly known as 2019-nCoV acute respiratory disease, is an infectious disease caused by SARS-CoV-2, a virus closely related to the SARS virus (1). The disease is the cause of the 2019?20 coronavirus outbreak (2). SARS-CoV-2 virus proteins include structural proteins, non-structural proteins and accessory factors. The structure of SARS-CoV-2 consists of the following: a spike protein (S), hemagglutinin-esterease dimer (HE), a membrane glycoprotein (M), an envelope protein (E) a nucleoclapid protein (N) and RNA. SARS-CoV-2 non-structural protein is ORF1ab that consists of 16 proteins (nsp1-nsp16), while accessory factors include ORF3a, ORF3b, ORF6, ORF7a, ORF7b, ORF8, ORF9b, ORF9c and ORF10.
ORF3a forms homotetrameric potassium sensitive ion channels (viroporin) and may modulate virus release. It up-regulates expression of fibrinogen subunits FGA, FGB and FGG in host lung epithelial cells. It induces apoptosis in cell culture and downregulates the type 1 interferon receptor by inducing serine phosphorylation within the IFN alpha-receptor subunit 1 (IFNAR1) degradation motif and increasing IFNAR1 ubiquitination (3).
- Gorbalenya. bioRxiv: 2020.
- Hui et al. Int J Infect Dis. 2020;91:264-266.
- Lu et al. PNAS USA. 2006; 103:12540-12545.
SARS-CoV-2 (COVID-19) ORF3a antibody is supplied in PBS containing 0.02% sodium azide.
1 mg/mL
Unconjugated
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Anti-SARS-CoV-2 (COVID-19) ORF3a antibody (9275) was raised against a peptide corresponding to 15 amino acids near the amino-terminus of SARS-CoV-2 (COVID-19) ORF3a protein.
The immunogen is located within the first 50 amino acids of the SARS-CoV-2 (COVID-19) ORF3a protein.
43740569
ORF3a protein
ORF3a
Severe acute respiratory syndrome coronavirus 2
Liquid
P0DTC3
1835921851
SARS-CoV-2 (COVID-19) ORF3a Antibody is affinity chromatography purified via peptide column.
Infectious Disease,COVID-19
P0DTC3
Optimal dilutions for each application to be determined by the researcher.