NSJ Bioreagents

CD28 Antibody

Product Code:
 
NSJ-V2987
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG2a, kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
204.12
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Functional Study
  • Immunofluorescence (IF)
Storage:
 
Store the CD28 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
1 / 2
Immunofluorescent staining of human Jurkat cells with CD28 antibody (green, clone 204.12) and Reddot nuclear stain (red).~
2 / 2
Flow cytometry testing of paraformaldehyde-fixed human Jurkat cells with CD28 antibody (clone 204.12); Red= isotyp
❮❯

Immunofluorescent staining of human Jurkat cells with CD28 antibody (green, clone 204.12) and Reddot nuclear stain (red).~
Flow cytometry testing of paraformaldehyde-fixed human Jurkat cells with CD28 antibody (clone 204.12); Red= isotyp

No additional charges, what you see is what you pay! *

CodeSizePrice
NSJ-V2987-20UG20 ug£264.00
Quantity:
NSJ-V2987-100UG100 ug£534.00
Quantity:
Prices exclude any Taxes / VAT
How to order with us   Contact us
Stay in control of your spending. These prices have no additional charges, not even shipping!
* Rare exceptions are clearly labelled (only 0.14% of items!).
Multibuy discounts available! Contact us to find what you can save.
This product comes from: United States.
Typical lead time: 10-14 working days.
Contact us for more accurate information.
  • Further Information
  • Documents
  • Show All

Further Information

Application Details :
Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml Functional studies (order BSA/sodium azide-free format)
Application Note:
Optimal dilution of the CD28 antibody should be determined by the researcher.
Description:
Recognizes a glycoprotein of 44-88kDa, which is identified as CD28. It is the critical T-cell co-stimulatory receptor which provides to the cell the important second activation signal by binding CD80 and CD86 that are expressed by antigen presenting cells. Besides its co-stimulation role, CD28 functions in preventing T-cells from anergic hyporesponsive state or from undergoing premature apoptotic cell death. CD28 is also expressed on human fetal NK cells and some NK cell lines, whereas on murine NK cells the CD28 expression is much broader.
Format :
Purified
Formulation :
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Immunogen:
Stimulated human leukocytes were used as the immunogen for the CD28 antibody.
Limitation:
This CD28 antibody is available for research use only.
Localization:
Cell surface
Purity:
Protein G affinity chromatography
Species Reactivity :
Human, Mouse
Uniprot #:
P10747