NSJ Bioreagents

PGK1 Antibody

Product Code:
 
NSJ-F54493
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
IgG2a, kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
1086CT10.2.1
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Aliquot the PGK1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
1 / 4
IHC testing of FFPE human kidney tissue with PGK1 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
2 / 4
IHC testing of FFPE human liver tissue with PGK1 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
3 / 4
Western blot testing of 1) human HEK293, 2) human A431 and 3) mouse brain lysate with PGK1 antibody. Predicted molecular weight ~44 kDa.
4 / 4
Flow cytometry testing of fixed and permeabilized human Jurkat cells with PGK1 antibody; Blue=isotype control, Green= PGK1 antibody.
❮❯

IHC testing of FFPE human kidney tissue with PGK1 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
IHC testing of FFPE human liver tissue with PGK1 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Western blot testing of 1) human HEK293, 2) human A431 and 3) mouse brain lysate with PGK1 antibody. Predicted molecular weight ~44 kDa.
Flow cytometry testing of fixed and permeabilized human Jurkat cells with PGK1 antibody; Blue=isotype control, Green= PGK1 antibody.

No additional charges, what you see is what you pay! *

CodeSizePrice
NSJ-F54493-0.05ML0.05 ml£260.00
Quantity:
NSJ-F54493-0.2ML0.2 ml£502.00
Quantity:
Prices exclude any Taxes / VAT
How to order with us   Contact us
Stay in control of your spending. These prices have no additional charges, not even shipping!
* Rare exceptions are clearly labelled (only 0.14% of items!).
Multibuy discounts available! Contact us to find what you can save.
This product comes from: United States.
Typical lead time: 10-14 working days.
Contact us for more accurate information.
  • Further Information
  • Documents
  • Show All

Further Information

Application Details :
Western blot: 1:500-1:2000, Flow cytometry: 1:25 (1x10e6 cells), Immunohistochemistry (FFPE): 1:25
Application Note:
The stated application concentrations are suggested starting points. Titration of the PGK1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
In addition to its role as a glycolytic enzyme, it seems that PGK-1 acts as a polymerase alpha cofactor protein (primer recognition protein).
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
Recombinant human protein was used as the immunogen for the PGK1 antibody.
Limitation:
This PGK1 antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Protein G affinity
Species Reactivity :
Human, Mouse
Uniprot #:
P00558