NSJ Bioreagents

KRT19 Antibody / Cytokeratin 19

Product Code:
 
NSJ-V3743
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG, kappa
Antibody Clonality:
 
Recombinant Antibody
Antibody Clone:
 
CTKN19-2R
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store the recombinant Cytokeratin 19 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
1 / 2
Western blot testing of human HepG2 antibody with Cytokeratin 19 antibody (clone CTKN19-2R). Predicted molecular weight ~43 kDa.~
2 / 2
IHC testing of FFPE human colon tissue with recombinant Cytokeratin 19 antibody (clone CTKN19-2R). Required
❮❯

Western blot testing of human HepG2 antibody with Cytokeratin 19 antibody (clone CTKN19-2R). Predicted molecular weight ~43 kDa.~
IHC testing of FFPE human colon tissue with recombinant Cytokeratin 19 antibody (clone CTKN19-2R). Required

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CodeSizePrice
NSJ-V3743-20UG20 ug£264.00
Quantity:
NSJ-V3743-100UG100 ug£534.00
Quantity:
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Further Information

Application Details :
Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1)
Application Note:
The stated application concentrations are suggested starting points. Titration of the recombinant Cytokeratin 19 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Description:
Keratin 19 is a type I keratin. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. Unlike its related family members, this smallest known acidic cytokeratin is not paired with a basic cytokeratin in epithelial cells. It is specifically found in the periderm, the transiently superficial layer that envelops the developing epidermis. [Wiki]
Format :
Purified
Formulation :
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Immunogen:
Human mammary epithelial organoids were used as the immunogen for the recombinant Cytokeratin 19 antibody.
Limitation:
This recombinant Cytokeratin 19 antibody is available for research use only.
Localization:
Cytoplasmic, cell membrane, secreted
Purity:
Protein A affinity chromatography
Species Reactivity :
Human
Uniprot #:
P08727