NSJ Bioreagents

S100B Antibody

Product Code:
 
NSJ-V3556
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG2a, kappa
Antibody Clonality:
 
Recombinant Antibody
Antibody Clone:
 
rS100B/1012
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store the recombinant S100B antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
1 / 3
IHC testing of FFPE human brain with recombinant S100B antibody (clone rS100B/1896). HIER: steam sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min.
2 / 3
IHC testing of FFPE human melanoma with recombinant S100B antibody (clone rS100B/1896). HIER: steam sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min.
3 / 3
Western blot testing of human brain tissue lysate with recombinant S100B antibody (clone rS100B/1896). Predicted molecular weight ~11 kDa.

IHC testing of FFPE human brain with recombinant S100B antibody (clone rS100B/1896). HIER: steam sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min.
IHC testing of FFPE human melanoma with recombinant S100B antibody (clone rS100B/1896). HIER: steam sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min.
Western blot testing of human brain tissue lysate with recombinant S100B antibody (clone rS100B/1896). Predicted molecular weight ~11 kDa.

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CodeSizePrice
NSJ-V3556-20UG20 ug£264.00
Quantity:
NSJ-V3556-100UG100 ug£534.00
Quantity:
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This product comes from: United States.
Typical lead time: 10-14 working days.
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Further Information

Application Details :
Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT,Western blot: 1-2ug/ml
Application Note:
The optimal dilution of the recombinant S100B antibody for each application should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Description:
S100 belongs to the family of calcium binding proteins. S100A and S100B proteins are two members of the S100 family. S100A is composed of an alpha and a beta chain whereas S100B is composed of two beta chains. This antibody is specific against an epitope located on the beta-chain (i.e. in S-100A and S-100B) but not on the alpha-chain of S-100 (i.e. in S-100A and S100A0). This antibody can be used to localize S-100A and S-100B in various tissue sections. S-100 protein has been found in normal melanocytes, Langerhans cells, histiocytes, chondrocytes, lipocytes, skeletal and cardiac muscle, Schwann cells, epithelial and myoepithelial cells of the breast, salivary and sweat glands, as well as in glial cells. Neoplasms derived from these cells also express S-100 protein, albeit non-uniformly. A large number of well-differentiated tumors of the salivary gland, adipose and cartilaginous tissue, and Schwann cell-derived tumors express S-100 protein. Almost all malignant melanomas and cases of histiocytosis X are positive for S-100 protein.
Format :
Purified
Formulation :
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Immunogen:
Full length human recombinant S100B protein was used as the immunogen for this recombinant S100B antibody.
Limitation:
This recombinant S100B antibody is available for research use only.
Localization:
Cytoplasmic, nuclear
Microarray Validated?:
Yes
Purity:
Protein G affinity chromatography
Species Reactivity :
Human
Uniprot #:
P04271