NSJ Bioreagents

ABCG2 Antibody

Product Code:
 
NSJ-F44284
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
 
Aliquot the ABCG2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
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Confocal immunofluorescent analysis of ABCG2 antibody with HepG2 cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used as a nuclear counterstain (blue).
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ABCG2 antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human placenta tissue
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ABCG2 antibody flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control (left histogram)
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Western blot analysis of ABCG2 antibody and 293 lysate
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Confocal immunofluorescent analysis of ABCG2 antibody with HepG2 cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used as a nuclear counterstain (blue).
ABCG2 antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human placenta tissue
ABCG2 antibody flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control (left histogram)
Western blot analysis of ABCG2 antibody and 293 lysate

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CodeSizePrice
NSJ-F44284-0.08ML0.08 ml£260.00
Quantity:
NSJ-F44284-0.4ML0.4 ml£502.00
Quantity:
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Further Information

Application Details :
Western blot: 1:1000,Immunofluorescence: 1:10-1:50,Flow Cytometry: 1:10-1:50,IHC (Paraffin): 1:50-1:100
Application Note:
Titration of the ABCG2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
ABCG2 is a membrane-associated protein included in the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the White subfamily. Alternatively referred to as a breast cancer resistance protein, this protein functions as a xenobiotic transporter which may play a major role in multi-drug resistance. It likely serves as a cellular defense mechanism in response to mitoxantrone and anthracycline exposure. Significant expression of this protein has been observed in the placenta, which may suggest a potential role for this molecule in placenta tissue.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 305-335 from the human protein was used as the immunogen for this ABCG2 antibody.
Limitation:
This ABCG2 antibody is available for research use only.
Purity:
Purified
Species Reactivity :
Human
Uniprot #:
Q9UNQ0