NSJ Bioreagents

BRAF Antibody

Product Code:
 
NSJ-F50794
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
 
Store at 4oC for up to one month. For long term, aliquot the BRAF antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
1 / 4
Western blot analysis of BRAF antibody and 293 cell lysate (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the B-RAF gene (2). Predicted size 85-95 kDa
2 / 4
Confocal immunofluorescent analysis of BRAF antibody with HeLa cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used as a nuclear counterstain (blue).
3 / 4
IHC analysis of FFPE human brain tissue stained with BRAF antibody
4 / 4
BRAF antibody flow cytometric analysis of HeLa cells (right histogram) compared to a negative control (left histogram).
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Western blot analysis of BRAF antibody and 293 cell lysate (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the B-RAF gene (2). Predicted size 85-95 kDa
Confocal immunofluorescent analysis of BRAF antibody with HeLa cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used as a nuclear counterstain (blue).
IHC analysis of FFPE human brain tissue stained with BRAF antibody
BRAF antibody flow cytometric analysis of HeLa cells (right histogram) compared to a negative control (left histogram).

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CodeSizePrice
NSJ-F50794-0.08ML0.08 ml£260.00
Quantity:
NSJ-F50794-0.4ML0.4 ml£502.00
Quantity:
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Further Information

Application Details :
Western blot: 1:1000,IHC (Paraffin): 1:10-1:50,Immunofluorescence: 1:10-1:50,Flow Cytometry: 1:10-1:50
Application Note:
The stated application concentrations are suggested starting amounts.Titration of the BRAF antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
BRAF is a protein kinase involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron. Phosphorylates MAP2K1, and thereby contributes to the MAP kinase signal transduction pathway. [UniProt]
Format :
Antigen affinity purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 424-453 from the human protein was used as the immunogen for this BRAF antibody.
Limitation:
This BRAF antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human
Species Reactivity (Predicted):
Mouse, Chicken
Uniprot #:
P15056