NSJ Bioreagents

Wee1 Antibody

Product Code:
 
NSJ-F46995
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
 
Aliquot the Wee1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
1 / 1
Wee1 antibody western blot analysis in WiDr lysate. Predicted molecular weight ~72 kDa.
❮❯

Wee1 antibody western blot analysis in WiDr lysate. Predicted molecular weight ~72 kDa.

No additional charges, what you see is what you pay! *

CodeSizePrice
NSJ-F46995-0.08ML0.08 ml£260.00
Quantity:
NSJ-F46995-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT
How to order with us   Contact us
Stay in control of your spending. These prices have no additional charges, not even shipping!
* Rare exceptions are clearly labelled (only 0.14% of items!).
Multibuy discounts available! Contact us to find what you can save.
This product comes from: United States.
Typical lead time: 10-14 working days.
Contact us for more accurate information.
  • Further Information
  • Documents
  • Show All

Further Information

Application Details :
Western blot: 1:1000
Application Note:
Titration of the Wee1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Wee1 may act as a negative regulator of entry into mitosis (G2 to M transition) by protecting the nucleus from cytoplasmically activated cyclin B1-complexed CDK1 before the onset of mitosis. Its activity increases during S and G2 phases and decreases at M phase when it is hyperphosphorylated. A correlated decrease in protein level occurs at M/G1 phase, probably due to its degradation. Specifically phosphorylates and inactivates cyclin B1-complexed CDK1 reaching a maximum during G2 phase and a minimum as cells enter M phase. Phosphorylation of cyclin B1-CDK1 occurs exclusively on 'Tyr-15' and phosphorylation of monomeric CDK1 does not occur (By similarity).
Format :
Antigen affinity purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 202-230 from the mouse protein was used as the immunogen for this Wee1 antibody.
Limitation:
This Wee1 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse
Species Reactivity (Predicted):
Rat
Uniprot #:
P47810