NSJ Bioreagents

ATG12 Antibody

Product Code:
 
NSJ-F41508
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
 
Aliquot the ATG12 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
1 / 3
Western blot analysis of ATG12 antibody and MCF-7 lysate.
2 / 3
Western blot analysis of ATG12 antibody and mouse brain tissue lysate
3 / 3
Mouse leukaemic monocyte macrophage cells treated with vinblastine for 1 hr. ATG12 antibody detected punctuate staining indicative of nascent autophagic vacuole structures.
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Western blot analysis of ATG12 antibody and MCF-7 lysate.
Western blot analysis of ATG12 antibody and mouse brain tissue lysate
Mouse leukaemic monocyte macrophage cells treated with vinblastine for 1 hr. ATG12 antibody detected punctuate staining indicative of nascent autophagic vacuole structures.

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CodeSizePrice
NSJ-F41508-0.08ML0.08 ml£260.00
Quantity:
NSJ-F41508-0.4ML0.4 ml£502.00
Quantity:
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Further Information

Application Details :
Western blot: 1:1000,Immunofluorescence: 1:50-1:100
Application Note:
Titration of the ATG12 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole).

APG12L/ATG12 is the human homolog of yeast APG12, a ubiquitin-activating enzyme E1-like protein essential for the conjugation system that mediates membrane fusion in autophagy.

Format :
Antigen affinity purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 1-50 from the human protein was used as the immunogen for this ATG12 antibody.
Limitation:
This ATG12 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse
Uniprot #:
O94817