NSJ Bioreagents

COX2 Antibody / PTGS2

Product Code:
 
NSJ-F54601
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Aliquot the Cyclooxygenase 2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
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Western blot testing of human HACAT cell lysate with Cyclooxygenase 2 antibody. Predicted molecular weight ~69 kDa.
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Western blot testing of human HeLa cell lysate with Cyclooxygenase 2 antibody. Predicted molecular weight ~69 kDa.
3 / 4
IHC testing of FFPE human uterus tissue with Cyclooxygenase 2 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
4 / 4
Immunofluorescent staining of human A549 cells with Cyclooxygenase 2 antibody (green) and DAPI nuclear stain (blue).
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Western blot testing of human HACAT cell lysate with Cyclooxygenase 2 antibody. Predicted molecular weight ~69 kDa.
Western blot testing of human HeLa cell lysate with Cyclooxygenase 2 antibody. Predicted molecular weight ~69 kDa.
IHC testing of FFPE human uterus tissue with Cyclooxygenase 2 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Immunofluorescent staining of human A549 cells with Cyclooxygenase 2 antibody (green) and DAPI nuclear stain (blue).

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CodeSizePrice
NSJ-F54601-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54601-0.4ML0.4 ml£502.00
Quantity:
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Further Information

Application Details :
Western blot: 1:500-1:2000, Immunohistochemistry (FFPE): 1:25, Immunofluorescence: 1:25
Application Note:
The stated application concentrations are suggested starting points. Titration of the Cyclooxygenase 2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Prostaglandin-endoperoxide synthase (PTGS), also known as cyclooxygenase, is the key enzyme in prostaglandin biosynthesis, and acts both as a dioxygenase and as a peroxidase. There are two isozymes of PTGS: a constitutive PTGS1 and an inducible PTGS2, which differ in their regulation of expression and tissue distribution. This gene encodes the inducible isozyme. It is regulated by specific stimulatory events, suggesting that it is responsible for the prostanoid biosynthesis involved in inflammation and mitogenesis.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 363-391 from the human protein was used as the immunogen for the Cyclooxygenase 2 antibody.
Limitation:
This Cyclooxygenase 2 antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Antigen affinity purified
Species Reactivity :
Human
Uniprot #:
P35354