ELK Biotechnology

Pig IGFBP3(Insulin Like Growth Factor Binding Protein 3) ELISA Kit

Product Code:
 
ELK5726
Product Group:
 
ELISA Kits
Supplier:
 
ELK Biotechnology
Regulatory Status:
 
RUO
Target Species:
 
Porcine (pig)
Application:
 
Enzyme-Linked Immunosorbent Assay (ELISA)
 

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CodeSizePrice
ELK5726-48T48T£359.00
Quantity:
ELK5726-96T96T£477.00
Quantity:
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This product comes from: China.
Typical lead time: 14-21 working days.
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Further Information

Alternative Names:
BP53; IBP3; Growth Hormone-Dependent Binding Protein; Acid Stable Subunit Of The 140 K IGF Complex
Assay Length:
3.5h
Assay Type:
Sandwich
Detection Range:
0.32-20 ng/mL
Sample Type:
Serum, plasma, tissue homogenates and other biological fluids.
Sensitivity:
0.138 ng/mL
Standard:
20 ng/mL
Test Principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pig IGFBP3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pig IGFBP3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pig IGFBP3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of Pig IGFBP3 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID:
P16611