NSJ Bioreagents

CYP7B1 Antibody

Product Code:
 
NSJ-F55002
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Aliquot the CYP7B1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
1 / 4
Western blot testing of human HeLa cell lysate with CYP7B1 antibody. Predicted molecular weight ~58 kDa.
2 / 4
IHC testing of FFPE human brain tissue with CYP7B1 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
3 / 4
Immunofluorescent staining of human HeLa cells with CYP7B1 antibody (green) and DAPI nuclear stain (blue).
4 / 4
Flow cytometry testing of human HeLa cells with CYP7B1 antibody; Blue=isotype control, Green= CYP7B1 antibody.
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Western blot testing of human HeLa cell lysate with CYP7B1 antibody. Predicted molecular weight ~58 kDa.
IHC testing of FFPE human brain tissue with CYP7B1 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Immunofluorescent staining of human HeLa cells with CYP7B1 antibody (green) and DAPI nuclear stain (blue).
Flow cytometry testing of human HeLa cells with CYP7B1 antibody; Blue=isotype control, Green= CYP7B1 antibody.

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CodeSizePrice
NSJ-F55002-0.08ML0.08 ml£260.00
Quantity:
NSJ-F55002-0.4ML0.4 ml£502.00
Quantity:
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Further Information

Application Details :
Western blot: 1:500-1:1000, Immunofluorescence: 1:10-1:50, Immunohistochemistry (FFPE): 1:50-1:100, Flow cytometry: 1:10-1:50 (1x10e6 cells)
Description:
CYP7B1 is a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This endoplasmic reticulum membrane protein catalyzes the first reaction in the cholesterol catabolic pathway of extrahepatic tissues, which converts cholesterol to bile acids. This enzyme likely plays a minor role in total bile acid synthesis, but may also be involved in the development of atherosclerosis, neurosteroid metabolism and sex hormone synthesis.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 252-281 from the human protein was used as the immunogen for the CYP7B1 antibody.
Limitation:
This CYP7B1 antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Purified
Species Reactivity :
Human
Uniprot #:
O75881