ProSci

CD11b Antibody [5C6 (recombinant version)], Rat IgG2b

Product Code:
 
PSI-24-188
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Antibody Isotype:
 
IgG2b kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
5C6 (recombinant version)
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry- Frozen Section (IHC-F)
Shipping:
 
Blue Ice
Storage:
 
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
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<b>Anti-CD11b staining of murine neutrophils.</b> <i>Ex vivo</i> murine (C57BL6/J) neutrophils from zymosan-elicited peritoneal exudate were stained with anti-CD11b antibody and analysed by flow-cytometry. Forward- and side-scatter was used to gate on neutrophil population (data not shown) and fluorescence intensity analysed. Left panel shows the signal obtained from cells pre-treated with Fc-blocking reagent and subsequently stained with anti-CD11b (hybridoma-derived antibody obtained from competitor- clone M1/70) directly conjugated to PerCP. Comparison to unstained cells shows that that cells analysed are predominantly CD11b-positive. Right panel shows staining first with anti-CD11b (24-188- clone 5C6 (recombinant version)) or isotype control (.1) followed by staining with fluorescently conjugated secondary antibody (goat anti-rat). As a further control- cells were also only stained with secondary antibody. Comparison of the staining pattern obtained using 24-188 compared to isotype- and secondary-only-control shows that the analysed cells are predominantly Cd11b-positive- agreeing with findings obtained using hybridoma-derived antibody.
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<b>Anti-CD11b staining of murine neutrophils.</b> <i>Ex vivo</i> murine (C57BL6/J) neutrophils from zymosan-elicited peritoneal exudate were stained with anti-CD11b antibody and analysed by flow-cytometry. Forward- and side-scatter was used to gate on neutrophil population (data not shown) and fluorescence intensity analysed. Left panel shows the signal obtained from cells pre-treated with Fc-blocking reagent and subsequently stained with anti-CD11b (hybridoma-derived antibody obtained from competitor- clone M1/70) directly conjugated to PerCP. Comparison to unstained cells shows that that cells analysed are predominantly CD11b-positive. Right panel shows staining first with anti-CD11b (24-188- clone 5C6 (recombinant version)) or isotype control (.1) followed by staining with fluorescently conjugated secondary antibody (goat anti-rat). As a further control- cells were also only stained with secondary antibody. Comparison of the staining pattern obtained using 24-188 compared to isotype- and secondary-only-control shows that the analysed cells are predominantly Cd11b-positive- agreeing with findings obtained using hybridoma-derived antibody.

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PSI-24-188-0.1mg0.1mg£850.00
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Further Information

Additional Names:
integrin alpha M, CD11 antigen-like family member B, CR-3 alpha chain, Cell surface glycoprotein MAC-1 subunit alpha, Leukocyte adhesion receptor MO1, Neutrophil adherence receptor
Buffer:
PBS with 0.02% Proclin 300.
Chimeric Use Statement:
This constant region of this antibody has been modified to bind to Protein A, allowing highly-specific purification of this antibody by Protein A-affinity column.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Fusion of spleen cells from AO rats immunised with Thioglycollate-elicted muine peritoneal macrophages (TPM) and the Y3 rat myeloma line.
NCBI Gene ID #:
3684
NCBI Official Name:
integrin subunit alpha M
NCBI Official Symbol:
ITGAM
NCBI Organism:
Homo sapiens
Research Area:
Immunology, Autophagy
Swissprot #:
P11215
User NOte:
For research use only.