ELISA Kit DIY Materials for ATPase, Ca++ Transporting, Cardiac Muscle, Slow Twitch 2 (ATP2A2)

Double-antibody Sandwich ELISA for Antigen Detection

0.055ng/mL

Main materials for "Do It(ELISA Kit) Yourself"

0.156-10ng/mL

Serum, plasma, tissue homogenates and other biological fluids.

Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate

The Abs in the kit have high sensitivity and excellent specificity for detection of ATPase, Ca++ Transporting, Cardiac Muscle, Slow Twitch 2 (ATP2A2). No significant cross-reactivity or interference between ATPase, Ca++ Transporting, Cardiac Muscle, Slow Twitch 2 (ATP2A2) and analogues was observed.

1. Coat the plates with 100uL per well of working solution of Capture Antibody.incubate overnight at 4?C or incubate at 37?C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 uL per well of working solution of Blocking Buffer. Incubate at 37?C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.