GENLISA™ Human Transmembrane Protease Serine 2 (TMPRSS2) ELISA

Please refer to the current IFU for the updated calibrator range and sensitivity.

Colorimetric, 450nm

The Human TransMembrane Protease, Serine 2, TMPRSS2 GENLISA ELISA kit is used as an analytical tool for quantitative determination of Human TransMembrane Protease, Serine 2, TMPRSS2 in serum, plasma and other biological samples. The GENLISA ELISA kits are used for assessing the specific biomarker in samples analytes which may be serum, plasma and cell culture supernatant as validated with the kit. The kit employs a sandwich ELISA technique which leads to a higher specificity and increased sensitivity compared to conventional competitive ELISA kits which employ only one antibody. Double antibodies are used in this kit. The method employs sandwich ELISA technique. Human TransMembrane Protease, Serine 2, TMPRSS2 Monoclonal antibodies are pre-coated onto microwells. Samples and standards are pipetted into microwells and Human TransMembrane Protease, Serine 2, TMPRSS2 present in the sample are bound by the antibodies. Biotin labeled TMPRSS2 antibody is added and followed by Streptavidin-HRP is pipetted and incubated to form a complex. After washing microwells in order to remove any non-specific binding, the substrate solution (TMB) is added to microwells and color develops proportionally to the amount of Human TransMembrane Protease, Serine 2, TMPRSS2 in the sample. Color development is then stopped by addition of stop solution. Absorbance is measured at 450 nm.

serum, cell cuture supernatant and plasma

1.20 ng/ml