Cloud-Clone Corp.

ELISA Kit for Anti-Glial Fibrillary Acidic Protein Antibody (Anti-GFAP)

Product Code:
 
AEA068Hu
Product Group:
 
ELISA Kits
Supplier:
 
Cloud-Clone Corp.
Regulatory Status:
 
RUO
 

No additional charges, what you see is what you pay! *

CodeSizePrice
AEA068Hu-24T24T£256.50£270.00
Special offer! 5% off Cloud-Clone Cancer Biomarker ELISA Kits. Read more here.
Quantity:
AEA068Hu-48T48T£426.55£449.00
Special offer! 5% off Cloud-Clone Cancer Biomarker ELISA Kits. Read more here.
Quantity:
AEA068Hu-96T96T£597.55£629.00
Special offer! 5% off Cloud-Clone Cancer Biomarker ELISA Kits. Read more here.
Quantity:
AEA068Hu-96T*596T*5£2,587.80£2,724.00
Special offer! 5% off Cloud-Clone Cancer Biomarker ELISA Kits. Read more here.
Quantity:
AEA068Hu-96T*1096T*10£4,862.10£5,118.00
Special offer! 5% off Cloud-Clone Cancer Biomarker ELISA Kits. Read more here.
Quantity:
Prices exclude any Taxes / VAT
Stay in control of your spending. These prices have no additional charges, not even shipping!
* Rare exceptions are clearly labelled (only 0.14% of items!).
Multibuy discounts available! Contact us to find what you can save.
This product comes from: China.
Typical lead time: 14-21 working days.
Contact us for more accurate information.
  • Further Information
  • Documents
  • Show All

Further Information

Alternative Names:
Intermediate Filament Protein
Assay Length:
2h, 30min
Assay Procedure Summary:
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 5 times;
5. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
6. Add 50µL Stop Solution. Read at 450nm immediately.
Detection Range:
3.12-200ng/mL
Item Name:
Glial Fibrillary Acidic Protein
Method:
Competitive Inhibition
Precision:
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Anti-Glial Fibrillary Acidic Protein Antibody (Anti-GFAP) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Anti-Glial Fibrillary Acidic Protein Antibody (Anti-GFAP) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Sample Type:
Serum, plasma and other biological fluids
Sensitivity:
The minimum detectable dose of this kit is typically less than 1.17ng/mL
Specificity:
This assay has high sensitivity and excellent specificity for detection of Anti-Glial Fibrillary Acidic Protein Antibody (Anti-GFAP).
No significant cross-reactivity or interference between Anti-Glial Fibrillary Acidic Protein Antibody (Anti-GFAP) and analogues was observed.
Stability:
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Test Principle:
The microtiter plate provided in this kit has been pre-coated with an antigen. Standards or samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP)-conjugated secondary antibody. After TMB substrate solution is added, those wells that contain Anti-Glial Fibrillary Acidic Protein Antibody (Anti-GFAP) will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of Anti-Glial Fibrillary Acidic Protein Antibody (Anti-GFAP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Uniprot ID:
P14136