MBL

Anti-FHL2 (Human) mAb

Product Code:
 
MBL-K0055-3
Product Group:
 
Primary Antibodies
Supplier:
 
MBL
Host Type:
 
Mouse
Antibody Isotype:
 
IgG2a
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
11-134
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
Applications:
  • ChIP
  • Immunocytochemistry (ICC)
  • Immunohistochemistry (IHC)
  • Immunoprecipitation (IP)
  • Western Blot (WB)
Shipping:
 
4°C
Storage:
 
-20°C
1 / 2
2 / 2
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MBL-K0055-3100 ug£350.00
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  • Further Information
  • Documents
  • References
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Further Information

Applications:
WB - 1-5 ug/mL IP - 3 ug / 200-300 uL of cell extract IHC - 1-5 ug/mL Heat treatment is necessary for paraffin embedded sections.
Background:
Proteins containing LIM domains (which are double zinc finger motifs implicated in protein binding) are important regulators of cell growth, cell differentiation, and remodeling of the cell cytoskeleton. Human Four-and-a-half LIM-only protein 2 (FHL2), also known as DRAL/Slim3 is a 32 kDa protein expressed predominantly in human heart and to a lesser extent in skeletal muscle, testis, and prostate epithelium. Since FHL2 is abundant in heart tissue, it may play a role in the regulation of myofibrillogenesis of heart via LIM-domain binding to focal adhesions. FHL2 has also been identified as a co-activator of the androgen receptor where it promotes androgen receptor transcriptional activity. Stimulation of the Rho signaling pathway induces translocation of FHL2 to the nucleus and subsequent activation of FHL2- and androgen receptor-dependent genes. FHL2 also acts as a trancriptional repressor in muscle cells and is involved in modulation of β-catenin-dependent transcription of Wnt-responsive genes.
Concentration:
1 mg/mL
Formulation:
100 ug IgG in 100 ul volume of PBS containing 50% glycerol, pH 7.2. No preservative iscontained.
Gene IDs:
Human: 2274 Mouse: 14200
Immunogen Translated:
Full-length recombinant human FHL2
Reactivity:
This antibody reacts with mouse FHL2.
Shelf Life:
1 year
Source:
This antibody was purified from hybridoma (clone 11-134) supernatant using protein-A Sepharose. This hybridoma was established by fusion of mouse myeloma cell SP2/0-Ag4 with Balb/c mouse splenocyte immunized with His-FHL2.
Target:
FHL2

Documents

References

1) Kahl, P., et al., Cancer Res. 66, 11341- 11347 (2006) 2) Sun, J., et al., Circ. Res. 99, 468-476 (2006) 3) Weinert, S., et al., J. Cell Biol. 173, 559-570 (2006) 4) Kang, D. E., et al., J. Biol. Chem. 280, 31537-31547 (2005) 5) Purcell, N. H., et al., Mol. Cell. Biol. 24, 1081-1095 (2004) 6) M?ller, J. M., et al., EMBO J. 21, 736-748 (2002) 7) M?ller, J. M., et al., EMBO J. 19, 359-369 (2000) Clone 11-134 is used in these references.