MBL

Anti-HA-tag mAb

Product Code:
 
MBL-M180-3
Product Group:
 
Primary Antibodies
Supplier:
 
MBL
Host Type:
 
Mouse
Antibody Isotype:
 
IgG2b κ
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
TANA2
Regulatory Status:
 
RUO
Applications:
  • Flow Cytometry
  • Immunocytochemistry (ICC)
  • Immunoprecipitation (IP)
  • Western Blot (WB)
Shipping:
 
4°C
Storage:
 
-20°C
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CodeSizePrice
MBL-M180-3200 ul£269.00
Quantity:
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This product comes from: United States.
Typical lead time: 10-14 working days.
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  • Further Information
  • Documents
  • References
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Further Information

Applications:
WB - 0.1 ug/mL (chemiluminescence detection system) IP - 2 ug/300 uL of cell extract from 3x106 cells FCM - 1 ug/mL (final concentration) ICC - 1 ug/mL
Background:
Epitope tag technique has widely been accepted technique that fuses an epitope peptide to a certain protein as a marker for gene expression. With this technique, the gene expression can be easily monitored on western blotting, immunoprecipitation, and immunofluorescence, utilizing with an antibody that recognizes such an epitope. HA-tag (YPYDVPDYA) sequence is widely used for the epitope tag, which corresponding to the partial peptide of Influenza hemagglutinin protein.
Concentration:
1 mg/mL
Formulation:
PBS containing 50% Glycerol (pH 7.2). No preservative is contained.
Immunogen Translated:
Synthetic peptide binding KLH, YPYDVPDYA (HA tag)
Reactivity:
This antibody reacts with N-terminal and C-terminal HA-tagged proteins.
Shelf Life:
1 year
Source:
Purified IgG from hybridoma supernatant
Target:
HA

Documents

References

1) Toyonaga, K., et al., PLoS One. 9, e88747 (2014) 2) Iguchi, M., et al., J. Biol. Chem. 288, 22019-22032 (2013) [WB]