Anti-Rb (Human) mAb

WB - 5-10 ug/mL (chemiluminescence detection system) IP - 1-5 ug / 200-300 uL of cell extract IHC - 5 ug/mL (Heat treatment required for paraffin)

Mutation of the retinoblastoma tumor suppressor gene alone is sufficient to cause retinoblastoma in humans, suggesting that it might play a role in the normal coordination of cell proliferation and cell death. Deletion or mutational inactivation of the retinoblastoma tumor suppressor protein (Rb) is correlated with the genesis of a variety of human cancers including retinoblastoma, osteosarcoma, and carcinomas of the breast, bladder, and lung. Rb protein is phosphorylated by cyclinD-Cdk4/Cdk6 and cyclinA/cyclinE-Cdk2 during the G1/S transition. This phosphorylation causes the inactivation of the growth inhibitory functions of Rb. Rb undergoes phosphorylation and functional inactivation, permitting the cell to proceed into late G1.

1 mg/mL

100 ug IgG in 100 ul PBS containing 50% glycerol, pH 7.2. Contains no preservatives.

Human: 5925 Mouse: 19645

Recombinant human Rb (612-928 a.a.)

This antibody reacts with human retinoblastoma gene product (110-115 kDa).

1 year

This antibody is precipitated from hybridoma (clone 3H9) supernatant using protein A Sepharose. This hybridoma was established by fusion of mouse myeloma cell P3U1 with Balb/c mouse splenocyte immunized with the recombinant human Rb protein corresponding to amino acids 612-928.

Rb