MBL

Anti-Atg14 (Human) pAb

Product Code:
 
MBL-PD026
Product Group:
 
Primary Antibodies
Supplier:
 
MBL
Host Type:
 
Rabbit
Antibody Isotype:
 
Affinity Purified Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunocytochemistry (ICC)
  • Immunoprecipitation (IP)
  • Western Blot (WB)
Shipping:
 
4°C
Storage:
 
-20°C
1 / 2
2 / 2
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CodeSizePrice
MBL-PD026100 ul£470.00
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  • Further Information
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Further Information

Applications:
WB - 1:500 (chemiluminescence detection system) IP - 5 uL/300 uL of cell extract from 3x106 cells
Background:
Autophagy is a process of intracellular bulk degradation in which cytoplasmic components including organelles are sequestered within double-membrane vesicles that deliver the contents to the lysosome/vacuole for degradation. Mammalian homologues of Atg14/Atg14L/BARKOR localizes on the isolation membrane and autophagosome, and it necessary for autophagosome formation. Atg14 binds to the PI3K complex (Beclin-1, Vps34, Vps15) and promotes the autophagosome formation.
Formulation:
100 ul volume of PBS containing 50% glycerol, pH 7.2. No preservative is contained.
Gene IDs:
Human: 22863 Mouse: 100504663 Rat: 305831
Immunogen Translated:
Recombinant human Atg14 (167-404 a.a.)
Reactivity:
This antibody reacts with human, mouse and rat Atg14 on Western blotting and Immunoprecipitation.
Shelf Life:
1 year
Source:
This antibody was purified from rabbit serum using affinity column. The rabbit was immunized with recombinant human Atg14 (167-404 aa).
Target:
Atg14

Documents

References

1) Matsunaga, K., et al., Nat. Cell Biol. 11, 385-396 (2009) 2) Zhong, Y., et al., Nat. Cell Biol. 11, 468-476 (2009) 3) Itakura, E., et al., Mol. Biol. Cell 19, 5360-5372 (2008) 4) Sun, Q., et al., PNAS 105, 19211-19216 (2008) This antibody is used in the reference number 3).