Elabscience

Anti-Human CD3-FITC/CD4-PE/CD45-PerCP-Cyanine5.5[OKT-3,RPA-T4,HI30]

Product Code:
 
E-AB-FC0003
Product Group:
 
Antibody Cocktails
Supplier:
 
Elabscience
Antibody Clone:
 
OKT-3,RPA-T4,HI30
Regulatory Status:
 
RUO
Target Species:
 
Human
Application:
 
Flow Cytometry
Shipping:
 
Ice packs
Storage:
 
Store at 2-8°C and protected from prolonged exposure to light. Do not freeze.
1 / 1
Human peripheral blood lymphocytes are stained with Anti-Human CD3-FITC/CD4-PE/CD45-PerCP-Cyanine5.5.

Human peripheral blood lymphocytes are stained with Anti-Human CD3-FITC/CD4-PE/CD45-PerCP-Cyanine5.5.

No additional charges, what you see is what you pay! *

CodeSizePrice
E-AB-FC0003-20Tests20Tests£133.00
Quantity:
E-AB-FC0003-100Tests100Tests£220.00
Quantity:
E-AB-FC0003-100Testsx2100Testsx2£321.00
Quantity:
Prices exclude any Taxes / VAT
Stay in control of your spending. These prices have no additional charges, not even shipping!
* Rare exceptions are clearly labelled (only 0.14% of items!).
Multibuy discounts available! Contact us to find what you can save.
This product comes from: China.
Typical lead time: 14-21 working days.
Contact us for more accurate information.
  • Further Information
  • Documents
  • Show All

Further Information

Background:
This product is a FCM antibody cocktail made up of FITC Anti-Human CD3 Antibody [Clone: OKT-3] (Mouse IgG2a, κ),PE Anti-Human CD4 Antibody [Clone: RPA-T4] (Mouse IgG1, κ) and PerCP/Cyanine5.5 Anti-Human CD45 Antibody [Clone: HI30] (Mouse IgG1, κ).; CD3 is a heterotetrameric protein consisting of a CD3γ, a CDδ and 2 CD3ε. It forms complex with TCR. OKT-3 recognize human CD3ε. Human CD3 is expressed on the surface of T cells and NKT cells.; CD4 is also called Leu-3 or T4. It's a single-chain type I transmembrane glycoprotein, mainly expressed on the surface of T cells, and monocytes/macrophages. In T cells, CD4 forms complex with TCR/CD3 and play important roles in T cell immunity. The target of HIV is CD4+ T cells. Reduction of CD4+ T cells is the main reason of defected immunity after HIV infection.; CD45 is a single-chain type I transmembrane glycoprotein. Except for erythrocytes and platelets, CD45 is expressed on nearly all of the hematopoietic cells with high level. It is a common marker for blood leukocytes. CD45 is a receptor type protein tyrosine phophatase and plays essential roles in B cell and T cells signaling.
Buffer:
Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Concentration:
5 uL/Test
Conjugation:
FITC,PE,PerCP/Cyanine5.5
Usage:
For whole blood samples, add 5 uL Anti-Human CD3-FITC/CD4-PE/CD45-PerCP-Cyanine5.5 Cocktail to 100 uL anticoagulant-treated blood sample. Mix and incubate the sample at 4°C in the dark for 30 min. Remove red blood cells with RBC lysis solution following the manufacturer's instruction. Wash the cell with cell staining buffer and discard the supernatant after centrifugation at 300 g for 5 min. Resuspend the cells with 200 uL cell staining buffer and load the sample on flow cytometer for detection. For other samples, 1x10^6 dissociated single cells are centrifuged at 300 g for 5 min with the supernatant discarded. Resuspend the cells with 100 uL cell staining buffer and add 5 uL Anti-Human CD3-FITC/CD4-PE/CD45-PerCP-Cyanine5.5 Cocktail. Mix and incubate the sample at 4°C in the dark for 30 min. Add cell staining buffer to each tube, centrifuge at 300 g for 5 min and discard the supernatant. Resuspend the cells with 200 uL cell staining buffer and load the sample on flow cytometer for detection.

Documents