Elabscience

NEK2 Polyclonal Antibody

Product Code:
 
E-AB-90632
Product Group:
 
Primary Antibodies
Supplier:
 
Elabscience
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Rat
Applications:
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Shipping:
 
Ice packs
Storage:
 
Store at -20°C. Avoid freeze/thaw cycles.
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Western blot analysis of extracts of HeLa cells using NEK2 Polyclonal Antibody at 1:1000 dilution.
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Immunohistochemistry of paraffin-embedded rat ovary using NEK2 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
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Western blot analysis of extracts of HeLa cells using NEK2 Polyclonal Antibody at 1:1000 dilution.
Immunohistochemistry of paraffin-embedded rat ovary using NEK2 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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E-AB-90632-60uL60uL£210.00
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E-AB-90632-120uL120uL£291.00
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E-AB-90632-200uL200uL£431.00
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Further Information

Abbreviation:
NEK2
Background:
This gene encodes a serine/threonine-protein kinase that is involved in mitotic regulation. This protein is localized to the centrosome, and undetectable during G1 phase, but accumulates progressively throughout the S phase, reaching maximal levels in late G2 phase. Alternatively spliced transcript variants encoding different isoforms with distinct C-termini have been noted for this gene.
Buffer:
PBS with 0.01% thiomersal,50% glycerol,pH7.3.
Calculated MW:
37kDa/44kDa/50kDa/51kDa
Concentration:
1mg/mL
Conjugation:
Unconjugated
Dilution:
WB 1:500-1:2000,IHC 1:50-1:200
Immunogen:
A synthetic peptide of human NEK2
ObservedMW:
52kDa
Purification method:
Affinity purification
Target Synonym:
NEK2;HsPK21;NEK2A;NLK1;PPP1R111;RP67
UNIProt ID:
P51955

Documents